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20/09/13
From 2013.igem.org
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(Created page with "==Miniprep from overnight broths== *DNA is isolated using Omega Miniprep kit and its protocol is followed ==Nanodropping the isolated DNA== {|border=1 |Sample||Volume ul||Concen...") |
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| + | {| style="color:#87EA00;background-color:#FFFFFF;" cellpadding="2" cellspacing="2" border="0" bordercolor="#000000" width="100%" align="center" | ||
| + | !align="center"|[[Team:Leicester|Home]] | ||
| + | !align="center"|[[Team:Leicester/Team|Team]] | ||
| + | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Leicester Official Team Profile] | ||
| + | !align="center"|[[Team:Leicester/Project|Project]] | ||
| + | !align="center"|[[Team:Leicester/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Leicester/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Leicester/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Leicester/Safety|Safety]] | ||
| + | !align="center"|[[Team:Leicester/Attributions|Attributions]] | ||
| + | |} | ||
| + | </div> | ||
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==Miniprep from overnight broths== | ==Miniprep from overnight broths== | ||
*DNA is isolated using Omega Miniprep kit and its protocol is followed | *DNA is isolated using Omega Miniprep kit and its protocol is followed | ||
Revision as of 10:08, 20 September 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Miniprep from overnight broths
- DNA is isolated using Omega Miniprep kit and its protocol is followed
Nanodropping the isolated DNA
| Sample | Volume ul | Concentration ng/ul | 260/280 | 260/230 |
| B5 |
Double digest of isolated DNA
- Digesting with EcorI-HF and PstI-HF to see if insert has been incorporated into DNA
Running on a 1.5% gel
- Gel pre-made on 18/09/13
- Running at 100V for 1.5 hours
