"
Team:Paris Saclay/Notebook/July/3
From 2013.igem.org
| Line 17: | Line 17: | ||
Tranformation of 07/02/13 works. We will do a Colony PCR of colonies. | Tranformation of 07/02/13 works. We will do a Colony PCR of colonies. | ||
|} | |} | ||
| - | |||
| - | |||
Colonies count for Bba_K1155000 : | Colonies count for Bba_K1155000 : | ||
| Line 50: | Line 48: | ||
[[File:PSPCR0307.jpg|400px]] | [[File:PSPCR0307.jpg|400px]] | ||
| - | ===='''2 - Culture of Bba_K1155000 | + | ===='''2 - Culture of Bba_K1155000 '''==== |
Sheng | Sheng | ||
| - | We made new cultures by streaking 2 colonies of 07/02/13 culture of Bba_K1155000 | + | We made new cultures by streaking 2 colonies of 07/02/13 culture of Bba_K1155000. We also did liquid culture of each one. |
We let culture at 37°C. | We let culture at 37°C. | ||
| + | |||
| + | ===='''Objective : obtaining Bba_K1155003, Bba_K1155007'''==== | ||
| + | |||
| + | ===='''1 - Culture of Bba_I732017, Bba_K592009 '''==== | ||
| + | |||
| + | Sheng | ||
| + | |||
| + | {| | ||
| + | | style="border:1px solid black;padding:5px;background-color:#DE;" | | ||
| + | Tranformation of 07/02/13 works. We will do new cultures. | ||
| + | |} | ||
| + | |||
| + | [[File:PsAmilCP0307.jpg|279px]][[File:PsLacZ0307.jpg|300px]] | ||
| + | |||
| + | We made new cultures by streaking 2 colonies of 07/02/13 culture of Bba_I732017, Bba_K592009. We also did liquid culture of each one. | ||
| + | We let culture at 37°C. | ||
| + | |||
{| border="1" align="center" | {| border="1" align="center" | ||
Revision as of 15:56, 25 September 2013
Contents |
Notebook : July 3
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining Bba_K1155000
1 - Colony PCR of Bba_K1155000
Abdou, Sheng, Zhou
|
Tranformation of 07/02/13 works. We will do a Colony PCR of colonies. |
Colonies count for Bba_K1155000 :
- Standard concentration :
- Bba_K1155000 : 0
- High concentration :
- Bba_K1155000 : 2
Primer and PCR :
VF2, VR, Pfnr_up, Pfnr_down are four oligos that we used for plasmid amplification. We used tree combinaisons VF/VR, VF/Pfnr_Down, Pfnr_Up/VR. If the promoter Pfnr insert PSB1C3 plasmid successfully, tree fragments with specific size will be amplified.
COLONIES PIQUEES DANS 20µL d'eau pour chaque colonie.
Used quantities :
- DNA : 2µL
- Mix : (it was divided in tubes for 4 different colonies for each oligo combinaison with 23µL of mix in each tube)
- VF or Pfnr_Up : 6µL
- VR or Pfnr_Down or VR : 6µL
- dNTP : 6µL
- Buffer Dream Taq : 30µL
- Dream Taq : 6µL
- H2O : 246µL
2 - Culture of Bba_K1155000
Sheng
We made new cultures by streaking 2 colonies of 07/02/13 culture of Bba_K1155000. We also did liquid culture of each one. We let culture at 37°C.
Objective : obtaining Bba_K1155003, Bba_K1155007
1 - Culture of Bba_I732017, Bba_K592009
Sheng
|
Tranformation of 07/02/13 works. We will do new cultures. |
We made new cultures by streaking 2 colonies of 07/02/13 culture of Bba_I732017, Bba_K592009. We also did liquid culture of each one. We let culture at 37°C.
| Previous day | Back to calendar | Next day |
