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Team:Paris Saclay/gel
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(Created page with "{{Team:Paris_Saclay/incl_debut_generique}} = '''Electrophoresis on gel''' = Gels are made with agarose in pounder with TAE 0.5X To make 1% gel: 1. Put 1g of agarose in pounder ...") |
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Gels are made with agarose in pounder with TAE 0.5X | Gels are made with agarose in pounder with TAE 0.5X | ||
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To make 1% gel: | To make 1% gel: | ||
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1. Put 1g of agarose in pounder | 1. Put 1g of agarose in pounder | ||
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2. Add TAE until 100mL | 2. Add TAE until 100mL | ||
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3. Boil until the solution become uniform | 3. Boil until the solution become uniform | ||
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4. Add BET with a final concentration of 200ng/µL | 4. Add BET with a final concentration of 200ng/µL | ||
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5. Pour gels in molds | 5. Pour gels in molds | ||
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6. Let agarose in suffusion at 60°C | 6. Let agarose in suffusion at 60°C | ||
Revision as of 20:13, 22 September 2013
Electrophoresis on gel
Gels are made with agarose in pounder with TAE 0.5X
To make 1% gel:
1. Put 1g of agarose in pounder
2. Add TAE until 100mL
3. Boil until the solution become uniform
4. Add BET with a final concentration of 200ng/µL
5. Pour gels in molds
6. Let agarose in suffusion at 60°C
Put the misted DNA with charged blue diluted 6X
