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Team:British Columbia/Notebook/Protocols
From 2013.igem.org
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===[[Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis|(Multi) Site Directed Mutagenesis]]=== | ===[[Team:British_Columbia/Notebook/Protocols/MultisiteMutagenesis|(Multi) Site Directed Mutagenesis]]=== | ||
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations. | ||
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| + | ===[[Team:British_Columbia/Notebook/Protocols/T4PNK|Oligonucleotide Phosphorylation]]=== | ||
| + | Phosphorylate oligonucleotides prior to ligation | ||
Revision as of 00:42, 23 September 2013
iGEM Home
Contents |
Protocols
Repeat-Spacer Array Assembly
Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides
Assembling Parts from Oligonucleotides
Assemble short parts from annealed oligonucleotides
(Multi) Site Directed Mutagenesis
Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.
Oligonucleotide Phosphorylation
Phosphorylate oligonucleotides prior to ligation
