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Team:INSA Toulouse/contenu/project/biological construction/riboregulation
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<h2 class="title2">Riboregulation System</h2> | <h2 class="title2">Riboregulation System</h2> | ||
| - | <p class="texte">In order to better control the expression of the recombinases, a | + | <p class="texte">In order to better control the expression of the recombinases, we design a riboregulation system to have well controlled promoters before our recombinases.</p> |
<img src="https://static.igem.org/mediawiki/2013/5/52/Riboregu_system.png" class="imgcontent" /> | <img src="https://static.igem.org/mediawiki/2013/5/52/Riboregu_system.png" class="imgcontent" /> | ||
<h3 class="title3">How does it work ?</h3> | <h3 class="title3">How does it work ?</h3> | ||
| - | <p class="texte">This is the basic system : Two promoters each followed by a RNA sequence</p> | + | <p class="texte">This is the basic system : Two inductible promoters each followed by a RNA sequence</p> |
<img src="https://static.igem.org/mediawiki/2013/1/10/How_does_it_work.png" class="imgcontent" /> | <img src="https://static.igem.org/mediawiki/2013/1/10/How_does_it_work.png" class="imgcontent" /> | ||
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<br> | <br> | ||
<br> | <br> | ||
| - | When P2 is activated and not P1, the red sequence will be transcripted. | + | When the promoter P2 is activated and not the P1, the red sequence will be transcripted. This red sequence possesses a similarity with the rbs sequence (in red). So when transcripted the red sequence will confine the rbs and prevent the recombinase from being translated. |
| - | This red sequence | + | |
</p> | </p> | ||
<img src="https://static.igem.org/mediawiki/2013/4/48/P2_activated.png" class="imgcontent" /> | <img src="https://static.igem.org/mediawiki/2013/4/48/P2_activated.png" class="imgcontent" /> | ||
| - | <p class="texte">When | + | <p class="texte">When both promoters are activated, both sequences (the blue and the red one) are transcripted. |
| - | + | The blue RNA sequence possesses a stronger similiraty with the red one. When transcripted the blue and the red sequences will match together and release the rbs to permit the translation of the recombinase.</p> | |
<img src="https://static.igem.org/mediawiki/2013/1/16/P1_and_P2_activated.png" class="imgcontent" /> | <img src="https://static.igem.org/mediawiki/2013/1/16/P1_and_P2_activated.png" class="imgcontent" /> | ||
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<h3 class="title3">Our strategy</h3> | <h3 class="title3">Our strategy</h3> | ||
| - | <p class="texte">These publications <a href="http://www.ncbi.nlm.nih.gov/pubmed/22454498">"Genetic switchboard for synthetic biology applications"</a> and <a href="http://www.ncbi.nlm.nih.gov/pubmed/15208640">"Engineered riboregulators enable post-transcriptional control of gene expression"</a>inspired us. In their work they present | + | <p class="texte">These publications <a href="http://www.ncbi.nlm.nih.gov/pubmed/22454498">"Genetic switchboard for synthetic biology applications"</a> and <a href="http://www.ncbi.nlm.nih.gov/pubmed/15208640">"Engineered riboregulators enable post-transcriptional control of gene expression"</a>inspired us. In their work they present one riboregulation system with this idea, we choose to design other riboswitches keeping some sequences from the original one and changing some nucleotides. It will aim use to have one riobregulation system specific for each input, then to do not have conflict. Furthemore, it will give use the possibility to characterize and compare the systems. </p> |
Revision as of 10:06, 27 September 2013
Biological Modules
Riboregulation System
In order to better control the expression of the recombinases, we design a riboregulation system to have well controlled promoters before our recombinases.
How does it work ?
This is the basic system : Two inductible promoters each followed by a RNA sequence
The blue and the red regions are regions which code for little RNA sequences.
When the promoter P2 is activated and not the P1, the red sequence will be transcripted. This red sequence possesses a similarity with the rbs sequence (in red). So when transcripted the red sequence will confine the rbs and prevent the recombinase from being translated.
When both promoters are activated, both sequences (the blue and the red one) are transcripted. The blue RNA sequence possesses a stronger similiraty with the red one. When transcripted the blue and the red sequences will match together and release the rbs to permit the translation of the recombinase.
Our strategy
These publications "Genetic switchboard for synthetic biology applications" and "Engineered riboregulators enable post-transcriptional control of gene expression"inspired us. In their work they present one riboregulation system with this idea, we choose to design other riboswitches keeping some sequences from the original one and changing some nucleotides. It will aim use to have one riobregulation system specific for each input, then to do not have conflict. Furthemore, it will give use the possibility to characterize and compare the systems.
