Team:USTC CHINA/Attributions

From 2013.igem.org

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         <h1>Overview</h1>
         <h1>Overview</h1>
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         <p>In our world, billions of people suffer from contagion, however, only part of them can be prevented by proper vaccine. Many disadvantages limit the usage of traditional vaccine especially in developing countries. </p>
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<img src="https://static.igem.org/mediawiki/igem.org/e/ec/2013ustc-china_traditional_vaccine_transport.jpg" width="400" height="300" />
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<div class="atfigure" align="center" style="width:400px;">Fig1. hard shipping in some area </div>
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<p>This year, our project focused on a revolutionary vaccine delivery. We chose bacillus subtilis as chassis to establish a transdermal vaccine fresh secreting band-aid which consists of four engineering b.subtilis, each of them carries a gene circuit independently.</p>
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<p>
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<img src="https://static.igem.org/mediawiki/2013/archive/e/ed/20130923171924!2013igemustc_Standardization.png" width="400" height="350" />
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In the last two months,thanks to our devotion and the support of various professors and scientific institutions ,our team achieved proud results.<br>
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<div class="atfigure" align="center" style="width:400px;">Fig2. block-based design </div>
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In the summer vacation,we  use  the gene material donated by scientific institution and individuals to  standardize and splice.we standardize and splice transdermal peptide  and gene circuits consists of various fusion protein of antigen.Meanwhile,we expressed TD1-GFP、TD1-HBsAg、TD1-LTB and all of expression of them was verified by in the E.coli. TD1-PA、TD1-HBsAg、TD1-Ag85b and TD1-LTB constructed circuits of the secretion vector,WB800N.Firstly,the transdermal experiment outside human body succeeded.Secondly,we also try to do experiment in living mice.Generally,we construct 16 new parts,we set a foundation for toolkit.Also our standardized BBa_K1074006 made a frame for other secretion expression researchers,theoretically users can use TD1 to merge any protein,to made the target protein have the function of secretion and transdermal permeation.<br>
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<p>By using an excellent transdermal peptide TD1, three of them could express a serious of fusion proteins(antigen, 2 kinds of adjuvants) which could penetrating the skin and work as traditional vaccine molecule.The fourth type of them is our “reporter” which could notify users whether the band-aid works well and when they could stick or tear off the patch. Moreover we designed a reliable suicide system in bacillus subtilis for the very first time in iGEM. So it would be OK if our users tear patches off and throw them away. Cheap and convenient, we just create a world without needle. </p>
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Thanks to the following individuals and institutions:
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1.gene donation
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Standardized antigen:Chinese Centre for Disease
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TD-1 short chain poly peptide:Professor Longping Wen
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2.theoretically support:
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Consultation of Bacillus subtilis:Professor Xin Run,Nanjing Agriculture University
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Consultation of transdermal  experiment:Professor Longping Wen
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Training of mice experiment:Professor Ting Yue
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3.Preliminary Partner:Hualan Biology Co.,ltd.
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Revision as of 10:14, 27 September 2013

Overview

In the last two months,thanks to our devotion and the support of various professors and scientific institutions ,our team achieved proud results.
In the summer vacation,we use the gene material donated by scientific institution and individuals to standardize and splice.we standardize and splice transdermal peptide and gene circuits consists of various fusion protein of antigen.Meanwhile,we expressed TD1-GFP、TD1-HBsAg、TD1-LTB and all of expression of them was verified by in the E.coli. TD1-PA、TD1-HBsAg、TD1-Ag85b and TD1-LTB constructed circuits of the secretion vector,WB800N.Firstly,the transdermal experiment outside human body succeeded.Secondly,we also try to do experiment in living mice.Generally,we construct 16 new parts,we set a foundation for toolkit.Also our standardized BBa_K1074006 made a frame for other secretion expression researchers,theoretically users can use TD1 to merge any protein,to made the target protein have the function of secretion and transdermal permeation.
Thanks to the following individuals and institutions: 1.gene donation Standardized antigen:Chinese Centre for Disease TD-1 short chain poly peptide:Professor Longping Wen 2.theoretically support: Consultation of Bacillus subtilis:Professor Xin Run,Nanjing Agriculture University Consultation of transdermal experiment:Professor Longping Wen Training of mice experiment:Professor Ting Yue 3.Preliminary Partner:Hualan Biology Co.,ltd.

Project

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