Team:Hong Kong HKUST/experiment/exp2
From 2013.igem.org
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<br><br><br><div id="slide"><center><h3 class="title">Blogging Synthetic Biology</h3></center><br> | <br><br><br><div id="slide"><center><h3 class="title">Blogging Synthetic Biology</h3></center><br> | ||
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- | <p id="yo"> | + | <p id="yo">BBa_ J176171 was planned to be used as a mammalian vector backbone for the promoters. After running PCR (Polymerase Chain Reaction) to amplify the FABP1 promoter and ligated it with GFP and the backbone, we did mutagenesis for the vector. Based on the gel photo, we concluded that the vector was easy to get degraded. Finally we figured out that BBa-J176171 was heat unstable and changed our plan.</p><br> |
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Revision as of 18:45, 27 September 2013
![](https://static.igem.org/mediawiki/igem.org/c/c7/BANNER1_%281%29.png)
Blogging Synthetic Biology
BBa_J176171
BBa_ J176171 was planned to be used as a mammalian vector backbone for the promoters. After running PCR (Polymerase Chain Reaction) to amplify the FABP1 promoter and ligated it with GFP and the backbone, we did mutagenesis for the vector. Based on the gel photo, we concluded that the vector was easy to get degraded. Finally we figured out that BBa-J176171 was heat unstable and changed our plan.