Team:Hong Kong HKUST/experiment/exp3

From 2013.igem.org

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<br><br><br><div id="slide"><center><h3 class="title">Blogging Synthetic Biology</h3></center><br>  
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<br><br><br><div id="slide"><center><h3 class="title">Protein Trafficking</h3></center><br>  
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<h3>Introduction</h3>
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<h1>Submission of BioBricks</h1>
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<p id="yo">For one of our human practice projects, our team investigated the current development of synthetic biology in some of the Asian countries. While doing research, we found out that synthetic biology is really a new field in science and that is not well-known in Asia. In fact, when some of our members tried to research information about synthetic biology in Cantonese, we could not find any webpages dedicated to share information about synthetic biology for the public in Hong Kong. To address this observation, we have decided to use an easily accessible platform called Google Blogger. This blog will be used as mean to introduce synthetic biology, share our experience and our project, and report current events related to synthetic biology. It will be written in Chinese and English so that most people in Hong Kong can read it easily and pay more attention to this new field in science. By providing holistic and informational blog posts, we aim at raising the public awareness to synthetic biology.</p><br>
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<p id="yo">The MLS was cloned from a commercial plasmid, pCMV/myc/mito (Invitrogen) by PCR. For the MLS BioBrick, we have submitted the MLS BioBrick in RFC 10 and RFC 25, the Freiburg format which allows protein fusion, to facilitate other team to fuse the MLS with other protein for purpose of introducing other protein into mitochondria. </p>
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<p id="yo">For characterization, MLS and green fluorescence protein was fused with constitutive mammalian CMV promoter. The promoter was cloned from pEGFP-N1 (Clonetech) in RFC10 format, since such part could not be found in partsregistry. The CMV cloned for our characterization construct was also submitted. The two construct for characterization, the CMV promoter – green fluorescent protein – polyadenylation sequence – pSB1C3 and CMV promoter – mitochondria leader sequence – green fluorescence protein – polyadenylation sequence – pSB1C3 composite parts are also submitted.</p><br>
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<h3>A Sneak Peek of Our Blog</h3><br>
<h3>A Sneak Peek of Our Blog</h3><br>
<center><img src="https://static.igem.org/mediawiki/2013/2/2e/Blog-cecilia.png" style="width:90%"></center><br>
<center><img src="https://static.igem.org/mediawiki/2013/2/2e/Blog-cecilia.png" style="width:90%"></center><br>

Revision as of 19:13, 27 September 2013




Protein Trafficking


Submission of BioBricks

The MLS was cloned from a commercial plasmid, pCMV/myc/mito (Invitrogen) by PCR. For the MLS BioBrick, we have submitted the MLS BioBrick in RFC 10 and RFC 25, the Freiburg format which allows protein fusion, to facilitate other team to fuse the MLS with other protein for purpose of introducing other protein into mitochondria.

For characterization, MLS and green fluorescence protein was fused with constitutive mammalian CMV promoter. The promoter was cloned from pEGFP-N1 (Clonetech) in RFC10 format, since such part could not be found in partsregistry. The CMV cloned for our characterization construct was also submitted. The two construct for characterization, the CMV promoter – green fluorescent protein – polyadenylation sequence – pSB1C3 and CMV promoter – mitochondria leader sequence – green fluorescence protein – polyadenylation sequence – pSB1C3 composite parts are also submitted.


A Sneak Peek of Our Blog




CLICK HERE TO VISIT OUR BLOG!

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