Team:Hong Kong HKUST/experiment/exp4

From 2013.igem.org

(Difference between revisions)
Line 210: Line 210:
</div>
</div>
-
<br><br><br><div id="slide"><center><h3 class="title">Blogging Synthetic Biology</h3></center><br>  
+
<br><br><br><div id="slide"><center><h3 class="title">Glyoxylate Shunt</h3></center><br>  
-
<h3>Introduction</h3>
+
<h1>Overview</h1>
-
<p id="yo">For one of our human practice projects, our team investigated the current development of synthetic biology in some of the Asian countries. While doing research, we found out that synthetic biology is really a new field in science and that is not well-known in Asia. In fact, when some of our members tried to research information about synthetic biology in Cantonese, we could not find any webpages dedicated to share information about synthetic biology for the public in Hong Kong. To address this observation, we have decided to use an easily accessible platform called Google Blogger. This blog will be used as mean to introduce synthetic biology, share our experience and our project, and report current events related to synthetic biology. It will be written in Chinese and English so that most people in Hong Kong can read it easily and pay more attention to this new field in science. By providing holistic and informational blog posts, we aim at raising the public awareness to synthetic biology.</p><br>
+
<p id="yo">Our artificial futile cycle is driven by the expression of two key enzymes in glyoxylate cycle. Since mammalian cells lack genes expressing glyoxylate shunt, we introduce these genes form <i>E. coli</i> and assemble them in a constitutive construct that will allow the expression of prokaryotic gene in eukaryotic cell. In addition to the constitutive system, we will also assemble an inducible construct which will allow tunable gene expression according to the concentration of fatty acid in the medium. We decided to introduce inducible system to prevent fatty acid deficiency in low concentration of plasma fatty acid and facilitate greater fatty acid uptake at a high circulating fatty acid levels. The inducible and constitutive system will be compared in terms of fatty acid uptake rate in a range concentration of fatty acid. </p>
 +
 
<h3>A Sneak Peek of Our Blog</h3><br>
<h3>A Sneak Peek of Our Blog</h3><br>
<center><img src="https://static.igem.org/mediawiki/2013/2/2e/Blog-cecilia.png" style="width:90%"></center><br>
<center><img src="https://static.igem.org/mediawiki/2013/2/2e/Blog-cecilia.png" style="width:90%"></center><br>

Revision as of 20:37, 27 September 2013




Glyoxylate Shunt


Overview

Our artificial futile cycle is driven by the expression of two key enzymes in glyoxylate cycle. Since mammalian cells lack genes expressing glyoxylate shunt, we introduce these genes form E. coli and assemble them in a constitutive construct that will allow the expression of prokaryotic gene in eukaryotic cell. In addition to the constitutive system, we will also assemble an inducible construct which will allow tunable gene expression according to the concentration of fatty acid in the medium. We decided to introduce inducible system to prevent fatty acid deficiency in low concentration of plasma fatty acid and facilitate greater fatty acid uptake at a high circulating fatty acid levels. The inducible and constitutive system will be compared in terms of fatty acid uptake rate in a range concentration of fatty acid.

A Sneak Peek of Our Blog




CLICK HERE TO VISIT OUR BLOG!

Retrieved from "http://2013.igem.org/Team:Hong_Kong_HKUST/experiment/exp4"