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Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
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| - | <font size="4"> ''' | + | <font size="4"> '''Phusion PCR (50ul reaction)''' </font> |
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| + | For each sample: | ||
| + | 35 ul ddH20 | ||
| + | 10 ul 5X Phusion buffer | ||
| + | 1.5 ul 10mM dNTP's | ||
| + | 1 ul each Primer (50 uM stock) | ||
| + | 1 ul appropriate diluted template DNA | ||
| + | 0.5 ul Phusion Polymerase | ||
Revision as of 20:46, 27 September 2013
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Cholera-Enzyme Protocols
Phusion PCR (50ul reaction)
For each sample: 35 ul ddH20 10 ul 5X Phusion buffer 1.5 ul 10mM dNTP's 1 ul each Primer (50 uM stock) 1 ul appropriate diluted template DNA 0.5 ul Phusion Polymerase |
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