Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols

(Difference between revisions)

Revision as of 21:09, 27 September 2013

Cholera - Enzyme Laboratory Protocols

Cholera Enzyme

March-April

Protocols-October

Cholera-Enzyme Protocols

Phusion PCR (50ul reaction)

For each sample add:

35 ul ddH2O
10 ul 5X Phusion buffer
1.5 ul 10mM dNTP's
1 ul each Primer (50 uM stock)
1 ul appropriate diluted template DNA
0.5 ul Phusion Polymerase

Taq polymerase PCR (50ul reaction)

For each sample add:

40 ul ddH2O
5 ul 10X TAQ buffer
1.5 ul 10 mM dNTP's
1 ul each Primer (50 uM stock)
1 ul appropriate diluted template DNA
0.5 ul TAQ Polymerase

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 : [[Team:BYU Provo/Notebook/Cholera - Enzymes/September-October|September]]
-: [[Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols|Protocols]]
+: [[Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols|Protocols-October]]
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