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Team:BYU Provo/Notebook/Cholera - Enzymes/Protocols
From 2013.igem.org
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===Any deviations from these protocols are listed in the notebook when they were used=== | ===Any deviations from these protocols are listed in the notebook when they were used=== | ||
Revision as of 21:48, 27 September 2013
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Cholera-Enzyme Protocols
Salt LB recipe
To 950 ml distilled H2O add
Adjust pH to 7.8
Fill to 1000 ml and autoclave
Phusion PCR (50ul reaction)
For each sample add:
Set the PCR machine to run the following cycle with the middle three phases being repeated 35 times
Hold at 4°C
Taq polymerase PCR (50ul reaction)
For each sample add:
Set the PCR machine to run the following cycle with the middle three phases being repeated 35 times
Hold at 4°C
===Any deviations from these protocols are listed in the notebook when they were used=== |
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