Team:British Columbia/Notebook/Protocols

From 2013.igem.org

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===[[Team:British_Columbia/Notebook/Protocols/GCMS|Gas chromatography-mass spectrometry for biosynthetic product detection]]===
===[[Team:British_Columbia/Notebook/Protocols/GCMS|Gas chromatography-mass spectrometry for biosynthetic product detection]]===
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GC-MS Protocol for assaying biosynthetic product production.
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===[[Team:British_Columbia/Notebook/Protocols/CombinatorialLibraries|Make Combinatorial Part Libraries]]===
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Make combinatorial part libraries from compatible parts

Revision as of 22:48, 27 September 2013

iGEM Home

Contents

Protocols

CRISPR Repeat-Spacer Array Assembly

Assemble combinatorial libraries of repeat-spacer-repeat arrays using oligonucleotides

Assembling Parts from Oligonucleotides

Assemble short parts from annealed oligonucleotides

(Multi) Site Directed Mutagenesis

Site directed mutagenesis. Protocol is directly amenable simultaneously making distant mutations.

Oligonucleotide Phosphorylation

Phosphorylate oligonucleotides prior to ligation

Electrocompetent Cell Production

Gas chromatography-mass spectrometry for biosynthetic product detection

GC-MS Protocol for assaying biosynthetic product production.

Make Combinatorial Part Libraries

Make combinatorial part libraries from compatible parts