TD1, Transdermal peptide
TD1 is a short synthetic peptide(ACSSSPSKHCG) identified by in vivo phage display, facilitated efficient transdermal protein delivery through intact skin...
Pgrac+RBS+SamyQ+TD1
+GFP
This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins...
PHT43
PHT43 is a E.coli-B.subtilis shuttle vector allowing high-level expression of secreted proteins in B.subtilis...
HBsAg
HBsAg is the surface antigen of the hepatitis B virus (HBV). It indicates current hepatitis B infection...
Ag85b
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin...
Protective Antigen Domain 4
Protective antigen (PA) is the central component of the three-part protein toxin secreted by Bacillus anthracis, the organism responsible for anthrax...
LTB
This eltB gene encodes for the Heat-labile enterotox(LT) in certain virulent strains of E.coli...
Pctc+RBS+
amilCP
Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. activated by endogenous sigma factor B ...
SdpA,SdpB,
SpbC
Killing factor of Bacillus subtilis ,SpbC Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway...
Pgrac+RBS+SDP
Spbc in SDP(sdpA,sdpB,spbC)(BBa_K1074009)operon is a killing factor of Bacillus subtilis...
PsdpRI+RBS
+SDP
We construct this gene circuit as our kill switch to kill the engineered Bacillus subtilis for the safety purpose...
Pgrac promoter
Prgac promoter(consisting of the groE promoter,the lacO operator and the gsiBSD sequence) allow induction by addition of ITPG...
promoter sdpR/I
This part is a sigma factor A-dependent promotor of the gene sdpR derived from the B.subtilis...
SamyQ
SamyQ is the signal peptide of the amyQ gene encoding an α-amylase in Bacillus subtilis WB800N...
RBS for Pgrac
An efficient RBS for promoter Pgrac(BBa_K1074012) in Bacillus subtilis.../p>
RBS for Pctc
This is an efficient RBS for promoter Pctc(BBa_K143010) in Bacillus subtilis...
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