Team:USTC CHINA/Project/Background
From 2013.igem.org
(Difference between revisions)
Line 70: | Line 70: | ||
<p align="justify">The key to the problems is to make a change in drug delivery methods and create an in situ expression system based on synthetic biology.<p> | <p align="justify">The key to the problems is to make a change in drug delivery methods and create an in situ expression system based on synthetic biology.<p> | ||
<h2>Design of Project<h2> | <h2>Design of Project<h2> | ||
- | <p align="justify"> This year, our project focused on a revolutionary vaccine delivery. We chose bacillus subtilis as chassis to establish a transdermal vaccine fresh secreting band-aid which consists of four engineering b.subtilis, each of them carries a gene circuit independently. By using an excellent transdermal peptide TD1, three of them could express a serious of fusion proteins(antigen, 2 kinds of adjuvants) which could penetrating the skin and work as traditional vaccine molecule. The fourth type of them is our “reporter” which could notify users whether the band-aid works well and when they could stick or tear off the patch. Moreover we designed a reliable suicide system in bacillus subtilis for the very first time in iGEM. Innovative and incredible, we plan to create a world without | + | <p align="justify"> This year, our project focused on a revolutionary vaccine delivery. We chose bacillus subtilis as chassis to establish a transdermal vaccine fresh secreting band-aid which consists of four engineering b.subtilis, each of them carries a gene circuit independently. By using an excellent transdermal peptide TD1, three of them could express a serious of fusion proteins(antigen, 2 kinds of adjuvants) which could penetrating the skin and work as traditional vaccine molecule. The fourth type of them is our “reporter” which could notify users whether the band-aid works well and when they could stick or tear off the patch. Moreover we designed a reliable suicide system in bacillus subtilis for the very first time in iGEM. Innovative and incredible, we plan to create a world without needles.<p> |
<h2>The Way We Realize T-vaccine<h2> | <h2>The Way We Realize T-vaccine<h2> | ||
<h3>1. Transdermal Peptide 1:<h3> | <h3>1. Transdermal Peptide 1:<h3> | ||
- | <p align="justify">When this idea first stroke us, it seemed amazing that our dream is so naïve and unpractical. Above all, how can we make antigen get through the skin barrier? We refer to various transdermal methods, like Iontophoresis, Sonophoresis, | + | <p align="justify">When this idea first stroke us, it seemed amazing that our dream is so naïve and unpractical. Above all, how can we make antigen get through the skin barrier? We refer to various transdermal methods, like Iontophoresis, Sonophoresis, Microneedles. They require special equipment and deal damage to skin. Our idea remained unrealistic until we find Transdermal peptide 1.<p> |
<p align="justify">TD-1 is an eleven amino acid peptide, which can greatly facilitate macromolecule transdermal delivery through intact skin. This was first discovered by professor Wen Longping in our university and the results were published on Nature biotechnology.<p> | <p align="justify">TD-1 is an eleven amino acid peptide, which can greatly facilitate macromolecule transdermal delivery through intact skin. This was first discovered by professor Wen Longping in our university and the results were published on Nature biotechnology.<p> | ||
TD1 Sequence<br> | TD1 Sequence<br> |
Revision as of 02:34, 28 September 2013