Team:Leeds/Notebook

From 2013.igem.org

(Difference between revisions)
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<div style="color:darkgreen;font-size:14pt">'''24th June'''</div>
<div style="color:darkgreen;font-size:14pt">'''24th June'''</div>
* ''We’ve moved into our office, deep in the basement of the research labs in the Miall building.
* ''We’ve moved into our office, deep in the basement of the research labs in the Miall building.
-
Our time has been taken up getting acquainted with our office ( and ourcoffee machine!) and roughing out the fine details of our protocol.''
+
* ''Our time has been taken up getting acquainted with our office ( and ourcoffee machine!) and roughing out the fine details of our protocol.''
* ''We’ve pulled together a combination of the teams various projects to create our final idea, we’re now looking at making a detector platform for physically binding substrates to activate a reporter. (Scintillating no?)
* ''We’ve pulled together a combination of the teams various projects to create our final idea, we’re now looking at making a detector platform for physically binding substrates to activate a reporter. (Scintillating no?)
-
Our team has been busy thinking of inventive assay design to characterise our biobricks; frantically trying to code our wiki and taking our team photos (important work)
+
* ''Our team has been busy thinking of inventive assay design to characterise our biobricks; frantically trying to code our wiki and taking our team photos (important work)''
''
''
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<br>
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<div style="color:darkgreen;font-size:14pt">'''28th June'''</div>
<div style="color:darkgreen;font-size:14pt">'''28th June'''</div>
* ''It's been a busy week here at Leeds iGem HQ. We've got a Biochemist and a Physicist (a rather handsome pair) frantically working on coding the wiki and creating images for the presentations and posters, it's gonna be beautiful (we hope)
* ''It's been a busy week here at Leeds iGem HQ. We've got a Biochemist and a Physicist (a rather handsome pair) frantically working on coding the wiki and creating images for the presentations and posters, it's gonna be beautiful (we hope)
-
We've had a team of biochemists (all 3 of them) hard at work designing primers and getting ready to steamroll into the lab next week.''
+
* ''We've had a team of biochemists (all 3 of them) hard at work designing primers and getting ready to steamroll into the lab next week.''
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<br>
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<div class="accordion-content">
<div class="accordion-content">
<div style="color:darkgreen;font-size:14pt">'''1st July'''</div>
<div style="color:darkgreen;font-size:14pt">'''1st July'''</div>
-
* ''With our primers in the post, we've been mass producing growth media and agar plates. We've resuspended and transformed a selection of the biobricks we plan on using, including YFP, GFP, INP, and some terminators. The wiki team have been locked in the basement and their not allowed out till it looks beautiful.''
+
* ''With our primers in the post, we've been mass producing growth media and agar plates. We've resuspended and transformed a selection of the biobricks we plan on using, including YFP, GFP, INP, and some terminators. * ''The wiki team have been locked in the basement and their not allowed out till it looks beautiful.''
<br>
<br>
<div style="color:darkgreen;font-size:14pt">'''2nd July'''</div>
<div style="color:darkgreen;font-size:14pt">'''2nd July'''</div>
-
* ''Today we've been writing out scripts for our tutorial videos, they'll be a series aimed at younger students with an introduction to synthetic biology, and a series aimed at Synthetic Biologists on things like Goldengate and Gibson assembly. Then we locked our token nanotechnologist into a lab with a couple of thousand pounds worth of microfluidics chips and we're hoping for the best.''
+
* ''Today we've been writing out scripts for our tutorial videos, they'll be a series aimed at younger students with an introduction to synthetic biology, and a series aimed at Synthetic Biologists on things like Goldengate and Gibson assembly. * ''Then we locked our token nanotechnologist into a lab with a couple of thousand pounds worth of microfluidics chips and we're hoping for the best.''
<br>
<br>
<div style="color:darkgreen;font-size:14pt">'''3rd July'''</div>
<div style="color:darkgreen;font-size:14pt">'''3rd July'''</div>
-
* ''Been a busy day; plasmid preps, glycerol stocks and further transformations of quorum sensing biobricks (pLux and LuxR), promoters and cyan fluorescent proteins. We then had a skype conference Cyrille Pauthenier, the advisor for the Evry iGem team. This rather charming fellow went into great detail on Goldengate and Goldenbrick assembly methods and their pros and cons. We ended our day doing soundtests for our educational videos; watch for the remix.''
+
* ''Been a busy day; plasmid preps, glycerol stocks and further transformations of quorum sensing biobricks (pLux and LuxR), promoters and cyan fluorescent proteins. * ''We then had a skype conference Cyrille Pauthenier, the advisor for the Evry iGem team. * ''This rather charming fellow went into great detail on Goldengate and Goldenbrick assembly methods and their pros and cons. We ended our day doing soundtests for our educational videos; watch for the remix.''
<br>
<br>
<div style="color:darkgreen;font-size:14pt">'''4th July'''</div>
<div style="color:darkgreen;font-size:14pt">'''4th July'''</div>

Revision as of 11:45, 4 July 2013

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Brief intro here, may change'

Contents

June 2013

Week 1

24th June
  • We’ve moved into our office, deep in the basement of the research labs in the Miall building.
  • Our time has been taken up getting acquainted with our office ( and ourcoffee machine!) and roughing out the fine details of our protocol.
  • We’ve pulled together a combination of the teams various projects to create our final idea, we’re now looking at making a detector platform for physically binding substrates to activate a reporter. (Scintillating no?)
  • Our team has been busy thinking of inventive assay design to characterise our biobricks; frantically trying to code our wiki and taking our team photos (important work)


25th June
  • Jeni, one of our first year biochemists has been working on her primer design, getting everything ready to start in the labs next week. We’ve been compiling our part list and getting our order ready; a buffer and bacteria shopping spree.


26th June
  • We’re trying to plan out our outreach programs, so watch this space. We’re going to be doing instructional videos on RC10 and golden brick assembly; as well as some more basic introduction to Synthetic Biology for younger students, alongside running some SynBio workshops for summer students that’ll be here at Leeds.


27th June
  • Today we've been working on our presentation; getting it ready for the YSB conference by running it past some of our lecturers; scary stuff. We've also been getting comfortable in the lab space, making up stacks of LB broth and preparing for some serious bacterial colonisation.


28th June
  • It's been a busy week here at Leeds iGem HQ. We've got a Biochemist and a Physicist (a rather handsome pair) frantically working on coding the wiki and creating images for the presentations and posters, it's gonna be beautiful (we hope)
  • We've had a team of biochemists (all 3 of them) hard at work designing primers and getting ready to steamroll into the lab next week.



July 2013

Week 2

1st July
  • With our primers in the post, we've been mass producing growth media and agar plates. We've resuspended and transformed a selection of the biobricks we plan on using, including YFP, GFP, INP, and some terminators. * The wiki team have been locked in the basement and their not allowed out till it looks beautiful.


2nd July
  • Today we've been writing out scripts for our tutorial videos, they'll be a series aimed at younger students with an introduction to synthetic biology, and a series aimed at Synthetic Biologists on things like Goldengate and Gibson assembly. * Then we locked our token nanotechnologist into a lab with a couple of thousand pounds worth of microfluidics chips and we're hoping for the best.


3rd July
  • Been a busy day; plasmid preps, glycerol stocks and further transformations of quorum sensing biobricks (pLux and LuxR), promoters and cyan fluorescent proteins. * We then had a skype conference Cyrille Pauthenier, the advisor for the Evry iGem team. * This rather charming fellow went into great detail on Goldengate and Goldenbrick assembly methods and their pros and cons. We ended our day doing soundtests for our educational videos; watch for the remix.


4th July
  • Whew, we will want to write a lot


5th July
  • End of the Week!



Week 3

8th July
  • We don't know yet


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Geneious, our fine sponsors and suppliers of software Bioline, our fine sponsors and suppliers of equipment Qiagen, our fine sponsors and suppliers of PCR kits
Bangs Laboratories, our fine sponsors and suppliers of silica beads
Leeds Homepage



Everything Below Here To Be Removed



This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

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