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Team:Freiburg/Project/toolkit
From 2013.igem.org
(Difference between revisions)
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<div id="final_checkbox_content"> | <div id="final_checkbox_content"> | ||
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| - | You have chosen to do a gene activation experiment using a non inducible Cas9-VP16 device. Therefor you first have to order the following plasmids from the <a href="http://parts.igem.org/Main_Page"> iGEM parts registry </a>: | + | You have chosen to do a gene activation experiment using a non inducible Cas9-VP16 device. Therefor you first have to order the following plasmids from the <a id="link" href="http://parts.igem.org/Main_Page"> iGEM parts registry </a>: |
</p> | </p> | ||
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<td> BBa_K1150017 </td> | <td> BBa_K1150017 </td> | ||
<td> CMV-HA-NLS-Cas9-NLS-BGH </td> | <td> CMV-HA-NLS-Cas9-NLS-BGH </td> | ||
| - | <td> <a href=""> order </a> </td> | + | <td> <a id="link" href=""> order </a> </td> |
| - | <td> <a href=""> genebank </a> </td> | + | <td> <a id="link" href=""> genebank </a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td> BBa_K1150020 </td> | <td> BBa_K1150020 </td> | ||
<td> CMV-HA-NLS-Cas9-L3-VP16-NLS-BGH </td> | <td> CMV-HA-NLS-Cas9-L3-VP16-NLS-BGH </td> | ||
| - | <td> <a href=""> order </a> </td> | + | <td> <a id="link" href=""> order </a> </td> |
| - | <td> <a href=""> genebank </a> </td> | + | <td> <a id="link" href=""> genebank </a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td> BBa_K1150034 </td> | <td> BBa_K1150034 </td> | ||
<td> crRNA - plasmid </td> | <td> crRNA - plasmid </td> | ||
| - | <td> <a href=""> order </a> </td> | + | <td> <a id="link" href=""> order </a> </td> |
| - | <td> <a href=""> genebank </a> </td> | + | <td> <a id="link" href=""> genebank </a> </td> |
</tr> | </tr> | ||
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<p> | <p> | ||
Note: <br> | Note: <br> | ||
| - | All plasmids are optimised for an expression in mammalian systems. The devices are also available containing an SV40 instead of an CMV promotor (have a look at our <a href="https://2013.igem.org/Team:Freiburg/parts"> parts </a> side). This system was tested mainly in CHO, HEK-293-T and HeLa cells. | + | All plasmids are optimised for an expression in mammalian systems. The devices are also available containing an SV40 instead of an CMV promotor (have a look at our <a id="link" href="https://2013.igem.org/Team:Freiburg/parts"> parts </a> side). This system was tested mainly in CHO, HEK-293-T and HeLa cells. |
</p> | </p> | ||
Revision as of 11:46, 29 September 2013
The uniCAS toolkit - Customize your experiments!
You want to have a maximum of activation or repression of your genes by a minimal effort? Then you have to use the uniCAS toolkit provided by the iGEM team Freiburg 2013. All you have to do is:
- Click yourself through the routine below
- Order the appropriate plasmids and oligos
- Conduct a minimal of cloning
- Start your personalized experiment
