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Team:Freiburg/Highlights
From 2013.igem.org
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By fusing <b>effector domains</b> to Cas9 we altered the properties it in various ways.</p><p> The <b>activation domain VP16</b> is able to activate transcription of genes. The fusion of the <b>transcriptional repressor domain KRAB</b> leads to synthetic repressor of gene expression. Specific <b>chromatin modification</b> was achieved by fusing a histone methyl transferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. </p> <p> </p> <p> | By fusing <b>effector domains</b> to Cas9 we altered the properties it in various ways.</p><p> The <b>activation domain VP16</b> is able to activate transcription of genes. The fusion of the <b>transcriptional repressor domain KRAB</b> leads to synthetic repressor of gene expression. Specific <b>chromatin modification</b> was achieved by fusing a histone methyl transferase G9a to dCas9. With this protein we are able to specifically repress endogenous gene expression. </p> <p> </p> <p> | ||
| - | We were able to induce our system on light stimulus. This was possible by using photorecetors of higher plants. | + | We were able to induce our system on <b>light stimulus</b>. This was possible by using photorecetors of higher plants. |
</p> <p> </p> <p> | </p> <p> </p> <p> | ||
By building a plasmid containing the necessary<b> RNAs</b> and <b>insertion sites</b> for targeting we created a modular, BioBrick compatible system for <b>multiple DNA targeting: The RNAimer.</b> | By building a plasmid containing the necessary<b> RNAs</b> and <b>insertion sites</b> for targeting we created a modular, BioBrick compatible system for <b>multiple DNA targeting: The RNAimer.</b> | ||
Revision as of 12:58, 29 September 2013
