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Team:TU-Delft/FinalApplication
From 2013.igem.org
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<b><font size="3">Procedure:</font></b><br> | <b><font size="3">Procedure:</font></b><br> | ||
<ol> | <ol> | ||
| - | <li>Dissolve the peptides. | + | <li>Dissolve the peptides. Signiferin was dissolved in water, in order to dissolve maximim 1M of NH4HCO3 was added in order to gain an end concentration of 5mM.</li> |
| - | <li> | + | <li>The peptide solutions were measured, using the nanodrop, and we determined to have an absorbance of 0.74 and 0.51 respectively.Using absorbance A= εcL=εc when L = 1cm [1] the concentration was measured to be </li> |
| - | <li></li> | + | |
| + | |||
| + | <li> In order to make diffusion measurable, we folded the membrane like a filter paper and placed it in the peptide solutiom</li> | ||
| + | |||
<li></li> | <li></li> | ||
| + | <li>The membrane is add</li> | ||
<br><br> | <br><br> | ||
</html> | </html> | ||
Revision as of 12:24, 30 September 2013
BandAid Application
Experiments
Aim: To check if it is feasible for the peptides to pass through the selected membranes (Cellulose Nitrate Membrane and PTFE Membrane).
Procedure:- Dissolve the peptides. Signiferin was dissolved in water, in order to dissolve maximim 1M of NH4HCO3 was added in order to gain an end concentration of 5mM.
- The peptide solutions were measured, using the nanodrop, and we determined to have an absorbance of 0.74 and 0.51 respectively.Using absorbance A= εcL=εc when L = 1cm [1] the concentration was measured to be
- In order to make diffusion measurable, we folded the membrane like a filter paper and placed it in the peptide solutiom
- The membrane is add
