"
Team:Leeds
From 2013.igem.org
m |
|||
| Line 10: | Line 10: | ||
[[File:Leeds sooperheader3.png|center|750px|awesome looking header|link=|frameless]] | [[File:Leeds sooperheader3.png|center|750px|awesome looking header|link=|frameless]] | ||
<br> | <br> | ||
| - | + | <font size="6">[[Team:Leeds/Project|The Microbeagle]]</font> | |
<br> | <br> | ||
| - | == | + | ==A biological system designed to detect the presence of pathogens in water samples== |
Micro-Beagle is a novel reporter system for E-coli that, as an iGEM first, has been designed to dynamically detect arbitrary target solids (including other cells) through a mechanism activated by cell surface binding. | Micro-Beagle is a novel reporter system for E-coli that, as an iGEM first, has been designed to dynamically detect arbitrary target solids (including other cells) through a mechanism activated by cell surface binding. | ||
| - | [[File:Leeds_NEWBS3image.png|400px|right| | + | [[File:Leeds_NEWBS3image.png|400px|right|An example of a low scale water purification system|link=http://www.purefilteredwater.com/bsf.htm|frameless]] |
Micro-Beagle was initially developed to address the need for a low cost, energy efficient and robust tool to be used to assess the effectiveness of current water purification systems. As the Micro-Beagle is intended to operate at a low cost it is ideal for use in low-income areas where water testing cannot be maintained at an acceptable frequency. | Micro-Beagle was initially developed to address the need for a low cost, energy efficient and robust tool to be used to assess the effectiveness of current water purification systems. As the Micro-Beagle is intended to operate at a low cost it is ideal for use in low-income areas where water testing cannot be maintained at an acceptable frequency. | ||
| Line 24: | Line 24: | ||
We foresee Micro-Beagle being adapted for both the detection of waterborne pathogens and a variety of other diagnostic applications, and we envision future multisensor Micro-Beagles in which diverse pathogens can be simultaneously and quantitatively measured from a single water sample | We foresee Micro-Beagle being adapted for both the detection of waterborne pathogens and a variety of other diagnostic applications, and we envision future multisensor Micro-Beagles in which diverse pathogens can be simultaneously and quantitatively measured from a single water sample | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
<br> | <br> | ||
==The Leeds 2013 iGEM team== | ==The Leeds 2013 iGEM team== | ||
| Line 36: | Line 31: | ||
Leeds is a city in West Yorkshire, in the North of England. It has strong historical ties to the wool industry, and is famous for the Tetley brand of bitter ale. | Leeds is a city in West Yorkshire, in the North of England. It has strong historical ties to the wool industry, and is famous for the Tetley brand of bitter ale. | ||
| - | The University of Leeds is a British Red Brick and member of the | + | The University of Leeds is a [http://en.wikipedia.org/wiki/Red_brick_university British Red Brick] and member of the Russell Group and was officially formed as an independent university via Royal Charter from King Edward VII in 1904. |
| - | + | ||
<br> | <br> | ||
}} | }} | ||
Revision as of 09:25, 2 October 2013
 | |||||||
 |  |  |  | ||||
 |  A biological system designed to detect the presence of pathogens in water samplesMicro-Beagle is a novel reporter system for E-coli that, as an iGEM first, has been designed to dynamically detect arbitrary target solids (including other cells) through a mechanism activated by cell surface binding.  Micro-Beagle was initially developed to address the need for a low cost, energy efficient and robust tool to be used to assess the effectiveness of current water purification systems. As the Micro-Beagle is intended to operate at a low cost it is ideal for use in low-income areas where water testing cannot be maintained at an acceptable frequency. Micro-Beagle is designed to also be a modular system, utilising Ice Nucleation Protein to express and position target-binding peptides on the cell surface. Target binding induces membrane stress that activates the Cpx signalling pathway, and Micro-Beagle thus utilises a promoter from this pathway (pCpxR) to initiate expression of a reporter protein, such as GFP. As a proof of concept, we have used silica beads as a model diagnostic target (a pathogen surrogate) and the silica-binding “Si4” sequence as the target-binding peptide. We foresee Micro-Beagle being adapted for both the detection of waterborne pathogens and a variety of other diagnostic applications, and we envision future multisensor Micro-Beagles in which diverse pathogens can be simultaneously and quantitatively measured from a single water sample
The Leeds 2013 iGEM team We are the Leeds 2013 iGEM team, We are a group of undergrads from various courses at the University of Leeds, all with a passion for synthetic biology! Leeds is a city in West Yorkshire, in the North of England. It has strong historical ties to the wool industry, and is famous for the Tetley brand of bitter ale. The University of Leeds is a [http://en.wikipedia.org/wiki/Red_brick_university British Red Brick] and member of the Russell Group and was officially formed as an independent university via Royal Charter from King Edward VII in 1904.
| ||||||
 |
| ||||||
| |||||||
| |||||||
