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Team:Freiburg/Notebook/modeling
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<p class="first_order"><a class="active" href="https://2013.igem.org/Team:Freiburg/Notebook/modeling"> Modeling </a></p> | <p class="first_order"><a class="active" href="https://2013.igem.org/Team:Freiburg/Notebook/modeling"> Modeling </a></p> | ||
<p class="first_order"><a href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation"> Standardization </a></p> | <p class="first_order"><a href="https://2013.igem.org/Team:Freiburg/Notebook/standardisation"> Standardization </a></p> | ||
| - | <p class="first_order"> <a id="link" href="https://2013.igem.org/Team:Freiburg/protocols"> | + | <p class="first_order"> <a id="link" href="https://2013.igem.org/Team:Freiburg/protocols"> Material and Methods </a> </p> |
Revision as of 11:33, 3 October 2013
Modeling Notebook
65000 cells per well were seeded. 24h before transfection.
The cells had been transfected at 21:30.
To generate time dependent data, one well was treated every 6h.
t0= 01:30
t1= 07:30
t2= 13:30
t3= 19:30
t4= 01:30
t5= 07:50
t6= 13:30
Every timepoint the supernant was taken for SEAP measurement and the cells were lysated with RIPA buffer for quantitative western blotting.
