Team:INSA Toulouse/contenu/project/biological construction

From 2013.igem.org

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   <p class="texte">The first question we had to face for the <i>E.calculus</i> project was: “how can we transpose an electronic device into a reasonable biological system?”
   <p class="texte">The first question we had to face for the <i>E.calculus</i> project was: “how can we transpose an electronic device into a reasonable biological system?”
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<br>The diagramm of an electronic full adder can be divided into two independant parts : Input and Output signals (A, B, C<sub>in</sub>, S, C<sub>out</sub>) and logic gates. Indeed, logic gates will ever be the same, but signals must be adaptable to considered applications and microorganisms.</p>
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<br>The diagramm of an electronic full adder can be divided into two independant parts : Input and Output signals (A, B, C<sub>in</sub>, S, C<sub>out</sub>) and logic gates (XOR, AND, OR). THe rational for doing this classification was: logic gates can be universal but input and output signals must be adaptable for diverse applications and microorganisms.</p>
    
    
   <img src="https://static.igem.org/mediawiki/2013/2/2c/Full-adder.png" class="imgcontent" />
   <img src="https://static.igem.org/mediawiki/2013/2/2c/Full-adder.png" class="imgcontent" />
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">Input</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/input">Input</span></h2>
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   <p class="texte">For the input, it was needed to use a signal that can easily represents an “ON and OFF” switch. The use of lights to represent the inputs was our first idea : a blue and a red light.</a></p>
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   <p class="texte">For the input, we needed a signal that could easily represents "ON" and "OFF" states. Light came as a natural solution because it is easily switchable to "ON" and "OFF" states and color can be varied to represent several inputs (A and B).</a></p>
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   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/output">Output</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/output">Output</span></h2>
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   <p class="texte">The output needed to be a signal that can be easily seen without any divice, something visual like a color.</a></p>
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   <p class="texte">The output needed to be a signal that can be easily seen without any complicated device or apparatus, something visual like the color of the organism bearing the full adder.</a></p>
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  <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/carry">Carry</span></h2>
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  <p class="texte">The carry,(C<sub>in</sub> and C<sub>out</sub>) out a molecule that can transmit a message from one colony to an other was essential. </a></p>
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  <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/riboregulation">Riboregulation System</span></h2>
 
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  <p class="texte">In order to better control the expression of the recombinases, a ribo regulation system has been add before the recombinases genes.</a></p>
 
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/logic_gates">Logic Gates</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/logic_gates">Logic Gates</span></h2>
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   <p class="texte">An electronic full adder is composed of 5 logic gates (2 XOR, 2 AND and 1 OR). To transpose these logic gates into biological gates, two publications published this year (2013) inspired us.</a></p>
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   <p class="texte">An electronic full adder is composed of 5 logic gates. Transcriptionally regulated logic gates exist and have already been described. However, a major breakthrough in Synthetic Biology appeared during 2013 with two publications related to recombination based logic gates. They inspired us and are the basis of our work.</a></p>
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  <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/carry">Carry</span></h2>
 
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  <p class="texte">To represent the carry, a molecule that can transmit a message from one colony to an other was essential. </a></p>
 
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/full_adder">Full Adder</span></h2>
   <h2 class="texte"> <span class="title2"><a href="https://2013.igem.org/Team:INSA_Toulouse/contenu/project/biological_construction/full_adder">Full Adder</span></h2>
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   <p class="texte">With a logic diagram, a full adder can be illustrated with 5 logic gates (2 XOR, 2 AND and 1 OR) A and B represent the two operands and Cin and Cout the carries. In our biological system, this diagramm represents the input part.</a></p>
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   <p class="texte">The initegration of the signals (input, ouput, carry) with the logic gates leads to the full adder. The description of the full biological adder can be found here.</a></p>

Revision as of 09:21, 3 October 2013

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Biological Modules

The first question we had to face for the E.calculus project was: “how can we transpose an electronic device into a reasonable biological system?”
The diagramm of an electronic full adder can be divided into two independant parts : Input and Output signals (A, B, Cin, S, Cout) and logic gates (XOR, AND, OR). THe rational for doing this classification was: logic gates can be universal but input and output signals must be adaptable for diverse applications and microorganisms.

Input

For the input, we needed a signal that could easily represents "ON" and "OFF" states. Light came as a natural solution because it is easily switchable to "ON" and "OFF" states and color can be varied to represent several inputs (A and B).

Output

The output needed to be a signal that can be easily seen without any complicated device or apparatus, something visual like the color of the organism bearing the full adder.

Carry

The carry,(Cin and Cout) out a molecule that can transmit a message from one colony to an other was essential.

Logic Gates

An electronic full adder is composed of 5 logic gates. Transcriptionally regulated logic gates exist and have already been described. However, a major breakthrough in Synthetic Biology appeared during 2013 with two publications related to recombination based logic gates. They inspired us and are the basis of our work.

Full Adder

The initegration of the signals (input, ouput, carry) with the logic gates leads to the full adder. The description of the full biological adder can be found here.

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