iGEM Evry 2013 achievements

Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum:

Here we detail how we fulfilled each of the iGEM medal requirements.

Gold Medal (requires any one of the following)

• (Improve the function of an existing BioBrick Part or Device) => LacO RFP
• Help any registered iGEM team
• Your project may have implications for the environment, security, safety and ethics and/or ownership and sharing.

Silver Medal

• We experimentally validate the aceB-sfGFP biobrick that we submitted to the iGEM Parts registry using a TECAN and microfluidics.
• We document the characterization of our Biobricks in "Main Page" section of the Parts Registry.
  • BBa_K1163102: AceB-sfGFP characterization
  • BBa_K1163103: AceB-LacI characterization
  • BBa_K1163105: FepA-sfGFP characterization
  • BBa_K1163106: FepA-LacI characterization
  • BBa_K1163108: Fes-sfGFP characterization
  • BBa_K1163109: Fes-LacI characterization
  • BBa_K1163111: YncE-sfGFP characterization
  • BBa_K1163112: YncE-LacI characterization
• Our project have implications for safety and ethics

Bronze Medal

• We register the team, have a great summer, and plan to have fun at the Regional Jamboree
• We complete and submit Judging form
• We make an awesome and accessible team Wiki. We manage to make it the more educational and scientific as we can !
• We will present a poster and a talk at the iGEM Jamboree
• We document xx BioBricks and submit 8 news BioBricks used in our project:
  • BBa_K1163102: AceB-sfGFP
  • BBa_K1163103: AceB-LacI
  • BBa_K1163105: FepA-sfGFP
  • BBa_K1163106: FepA-LacI
  • BBa_K1163108: Fes-sfGFP
  • BBa_K1163109: Fes-LacI
  • BBa_K1163111: YncE-sfGFP
  • BBa_K1163112: YncE-LacI

And more precisely ?!

Biology

• We design and create 4 Iron sensing systems, that could also works with an invertor !
• We design an Iron chelating system and isolate genes of the enterobactins biosynthesis pathway !
• We use TECAN and microfluidic to analyse our systems and show that they work !
• We created a pill adapted to our project and that fulfilled basic requirements of the European Pharmacopeia !

Model

Human Practices

• We make a series of interviews and discussion with experts on iron overload diseases.
• We make reflexion about the safety of potential consequences of our bacteria, including a consequence tree.
• We had discussion with hemochromatosis patient and held a seminar on iron overload and on our project on 18 September 2013.
  Scientists working on iron overload, patients, and students attended to it !
• We make a survey about hemochromatosis and about our potential treatment.
  We received and analyzed more than 250 answers from patients !
• We submitted an extensive report of our conclusions about synthetic biology , which is the fruit of our long reflection we conducted over the summer.