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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
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<li><a href="/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">Gelelectrophoresis</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">Gelelectrophoresis</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/inoue">Inoue buffer</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/inoue">Inoue buffer</a></li> | ||
| + | <li><a href="/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">Chemo-competent <i>E. coli</i> cells</li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"><i>E. coli</i> transformation</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"><i>E. coli</i> transformation</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/pgem-ligation">pGEM Ligaton - ligation for TA-cloning of PCR products</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/pgem-ligation">pGEM Ligaton - ligation for TA-cloning of PCR products</a></li> | ||
Revision as of 23:56, 3 October 2013
Protocols
General protocols and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- DNA-ladder-buffer mix
- Gelelectrophoresis
- Inoue buffer
- Chemo-competent E. coli cells
- E. coli transformation
- pGEM Ligaton - ligation for TA-cloning of PCR products
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini-Prep
- Gelextraction
- Colony-PCR
Saccharomyces cerevisiae specific protocols
