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Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
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<div id="actualContent"> | <div id="actualContent"> | ||
| - | <h3>General | + | <h3>General and <i>E. coli</i> protocols</h3> |
<ul> | <ul> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/sob-medium">SOB medium</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/sob-medium">SOB medium</a></li> | ||
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<p> </p> | <p> </p> | ||
| - | <h3><i>Saccharomyces cerevisiae</i> specific protocols</h3> | + | <h3><i>Saccharomyces cerevisiae</i>-specific protocols</h3> |
<ul> | <ul> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/ypd">YPD medium</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/ypd">YPD medium</a></li> | ||
Revision as of 03:51, 4 October 2013
Protocols
General and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- DNA-ladder-buffer mix
- Gelelectrophoresis
- Inoue buffer
- Chemo-competent E. coli cells
- E. coli transformation
- pGEM Ligaton - ligation for TA-cloning of PCR products
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini Prep
- Gel Extraction
- Colony-PCR
Saccharomyces cerevisiae-specific protocols
