"
Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
| Line 28: | Line 28: | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">Chemo-competent <i>E. coli</i> cells</li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/chemo-competentcells">Chemo-competent <i>E. coli</i> cells</li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"><i>E. coli</i> transformation</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/trafo"><i>E. coli</i> transformation</a></li> | ||
| - | + | <!-- <li><a href="/Team:Tuebingen/Notebook/Protocols/pgem-ligation">pGEM Ligaton - ligation for TA-cloning of PCR products</a></li> --> | |
<li><a href="/Team:Tuebingen/Notebook/Protocols/ligation">Ligation of digested parts and vectors</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/ligation">Ligation of digested parts and vectors</a></li> | ||
<li><a href="/Team:Tuebingen/Notebook/Protocols/glycerol-stocks">Glycerol stocks</a></li> | <li><a href="/Team:Tuebingen/Notebook/Protocols/glycerol-stocks">Glycerol stocks</a></li> | ||
Revision as of 12:13, 4 October 2013
Protocols
General and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- DNA-ladder-buffer mix
- Gelelectrophoresis
- Inoue buffer
- Chemo-competent E. coli cells
- E. coli transformation
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini Prep
- Gel Extraction
- Colony-PCR
- Handling of the Tecan reader
Saccharomyces cerevisiae-specific protocols
