Team:Paris Saclay/Notebook/September/26

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We tested our PCR machine and it works as expected.
We tested our PCR machine and it works as expected.
 +
 +
Used quantities :
 +
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* pIMI018 (DNA) : 4µL
 +
* Primer aacF (10 µM) : 10µL
 +
* Primer aacR (10 µM) : 10µL
 +
* dXTP : 2 µL
 +
* Buffer 10x : 10µL
 +
* Q solution : 20µL
 +
* Taq (Qiagen : 0.8µL
 +
* H2O : 44µL
 +
 +
[[File:PsPSPCR.jpg|400px]]
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Revision as of 13:27, 4 October 2013

Navigation Home Team Project Labwork Notebook Human practices

Notebook : September 12

Lab work

Paris-Saclay PCR

Damir

We tested our PCR machine and it works as expected.

Used quantities :

  • pIMI018 (DNA) : 4µL
  • Primer aacF (10 µM) : 10µL
  • Primer aacR (10 µM) : 10µL
  • dXTP : 2 µL
  • Buffer 10x : 10µL
  • Q solution : 20µL
  • Taq (Qiagen : 0.8µL
  • H2O : 44µL

PsPSPCR.jpg

Pspcr test.png
  • Well 1 : 15µL DNA Ladder
  • Wells 2 & 3 : PCR products amplified with the PS-PCR with oil in the wells, 15µl
  • Well 4 : PCR product amplified with the PSPCR without oil in the well, 15µl
  • Well 5 : Control PCR product amplified with a commercial thermal cycler, 5µl

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