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Team:Evry/Sensor
From 2013.igem.org
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We constructed iron sensors by combining 3 genetic parts: an E. coli promoter with a Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below. | We constructed iron sensors by combining 3 genetic parts: an E. coli promoter with a Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below. | ||
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<div align='center'><img src="https://static.igem.org/mediawiki/2013/1/12/ColiSensor.png" width="75%"/></div> | <div align='center'><img src="https://static.igem.org/mediawiki/2013/1/12/ColiSensor.png" width="75%"/></div> | ||
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<b>Fig</b> Diagram of a genetic iron sensor. Iron bind the Ferric Uptake Regulator (Fur) to form a complex that binds the Fur box in the promoter, here shown as the aceB promoter. Once the iron-Fur complex is bound, it represses transcription of the target gene GFP. | <b>Fig</b> Diagram of a genetic iron sensor. Iron bind the Ferric Uptake Regulator (Fur) to form a complex that binds the Fur box in the promoter, here shown as the aceB promoter. Once the iron-Fur complex is bound, it represses transcription of the target gene GFP. | ||
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<div align='center'><img src="https://static.igem.org/mediawiki/2013/7/7b/P1-GFP.png" width="75%"/></div> | <div align='center'><img src="https://static.igem.org/mediawiki/2013/7/7b/P1-GFP.png" width="75%"/></div> | ||
<div align='center'><img src="https://static.igem.org/mediawiki/2013/f/f3/P1-Nat_Prom.png" width="58%"/></div> | <div align='center'><img src="https://static.igem.org/mediawiki/2013/f/f3/P1-Nat_Prom.png" width="58%"/></div> | ||
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| + | <b>Fig</b> Construction of an iron-responsive genetic element by fusing a Fur-regulated promoter with a reporter gene. Promoter-reporter fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning. | ||
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<table cellpadding="10" cellspacing="0" align='center' border="1"> | <table cellpadding="10" cellspacing="0" align='center' border="1"> | ||
Revision as of 09:57, 4 October 2013
Sensor
We constructed iron sensors by combining 3 genetic parts: an E. coli promoter with a Fur binding site, the sfGFP fluorescent reporter, and a transcriptional terminator. These iron sensing elements were cloned in Biobrick compatible format as shown below.
Fig Diagram of a genetic iron sensor. Iron bind the Ferric Uptake Regulator (Fur) to form a complex that binds the Fur box in the promoter, here shown as the aceB promoter. Once the iron-Fur complex is bound, it represses transcription of the target gene GFP.
Fig Construction of an iron-responsive genetic element by fusing a Fur-regulated promoter with a reporter gene. Promoter-reporter fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.
| NAME | FIGURE | DESCRIPTION |
|---|---|---|
|
E. coli promoter with Fur binding site |
|
iron-Fur complex binds promoter to repress expression |
|
sfGFP |
|
Fluorescent reporter gene |
|
Terminator |
|
terminator to stop transcription |
|
Plasmid |
|
Biobrick-compatible plasmid backbone |
