"
Team:Evry/Sensor
From 2013.igem.org
| Line 7: | Line 7: | ||
<h1>Iron Sensor</h1> | <h1>Iron Sensor</h1> | ||
| - | <p> | + | <div align='center'><p> |
We constructed iron-responsive biosensors by combining 3 genetic parts: an E. coli promoter with a Ferric Uptake Regulator (Fur) binding site, a fluorescent reporter (sfGFP), and a transcriptional terminator. These sensors respond to ambient iron by using the <a href="https://2013.igem.org/Team:Evry/Project_FUR">Fur system</a> to repress a target gene. | We constructed iron-responsive biosensors by combining 3 genetic parts: an E. coli promoter with a Ferric Uptake Regulator (Fur) binding site, a fluorescent reporter (sfGFP), and a transcriptional terminator. These sensors respond to ambient iron by using the <a href="https://2013.igem.org/Team:Evry/Project_FUR">Fur system</a> to repress a target gene. | ||
| - | </p> | + | </p></div> |
<br> | <br> | ||
<br> | <br> | ||
Revision as of 18:54, 4 October 2013
Iron Sensor
We constructed iron-responsive biosensors by combining 3 genetic parts: an E. coli promoter with a Ferric Uptake Regulator (Fur) binding site, a fluorescent reporter (sfGFP), and a transcriptional terminator. These sensors respond to ambient iron by using the Fur system to repress a target gene.
Fig 1 Diagram of a genetic iron sensor. Iron binds the Ferric Uptake Regulator (Fur) to form a complex with high affinity for the Fur box in the promoter, here shown as the aceB promoter. Once the iron-Fur complex is bound to the promoter, it represses transcription of the target gene GFP. GFP expression is thus negatively correlated with iron availability.
Fig 2 Construction of an iron-responsive genetic element by fusing a Fur-regulated promoter with a reporter gene. Promoter-reporter fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.
Table I Genetic elements used to make iron-responsive sensors.
| NAME | FIGURE | DESCRIPTION |
|---|---|---|
|
E. coli promoter with Fur binding site |
|
iron-Fur complex binds promoter to repress expression |
|
sfGFP |
|
Fluorescent reporter gene |
|
Terminator |
|
terminator to stop transcription |
|
Plasmid |
|
Biobrick-compatible plasmid backbone |
