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Team:Evry/Chelator
From 2013.igem.org
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<img src="https://static.igem.org/mediawiki/2013/c/cc/P3-Ent1.png" width="100%"/> | <img src="https://static.igem.org/mediawiki/2013/c/cc/P3-Ent1.png" width="100%"/> | ||
</div> | </div> | ||
| + | <p> | ||
| + | <b>Fig 1.</b> Iron-responsive genetic inverter. The iron-Fur complex binds to the Fur site, here in the aceB promoter, to repress transcription of the lacI gene. In the absence of LacI, the RFP reporter is expressed. | ||
| + | </p> | ||
Assembly of enterobactin chelator genes entA-entF into a single operon | Assembly of enterobactin chelator genes entA-entF into a single operon | ||
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<img src="https://static.igem.org/mediawiki/2013/6/6d/P3-Ent2.png" width="100%"/> | <img src="https://static.igem.org/mediawiki/2013/6/6d/P3-Ent2.png" width="100%"/> | ||
</div> | </div> | ||
| - | + | <p> | |
| + | <b>Fig 1.</b> Iron-responsive genetic inverter. The iron-Fur complex binds to the Fur site, here in the aceB promoter, to repress transcription of the lacI gene. In the absence of LacI, the RFP reporter is expressed. | ||
| + | </p> | ||
<table cellpadding="10" cellspacing="0" align='center' border="1"> | <table cellpadding="10" cellspacing="0" align='center' border="1"> | ||
<tr> | <tr> | ||
Revision as of 19:08, 4 October 2013
Iron Chelator
Fig 1. Iron-responsive genetic inverter. The iron-Fur complex binds to the Fur site, here in the aceB promoter, to repress transcription of the lacI gene. In the absence of LacI, the RFP reporter is expressed.
Assembly of enterobactin chelator genes entA-entF into a single operon
Fig 1. Iron-responsive genetic inverter. The iron-Fur complex binds to the Fur site, here in the aceB promoter, to repress transcription of the lacI gene. In the absence of LacI, the RFP reporter is expressed.
| NAME | FIGURE | Description |
|---|---|---|
|
Lac promoter |
|
first gene for enterobactin sythesis |
|
RBS + EntA |
|
second gene for enterobactin sythesis |
|
RBS + EntB |
|
third gene for enterobactin sythesis |
|
RBS + EntC |
|
fourth gene for enterobactin sythesis |
|
RBS + EntD |
|
fifth gene for enterobactin sythesis |
|
RBS + EntE |
|
sixth gene for enterobactin sythesis |
|
RBS + EntF |
|
Comment... |
|
Terminator |
|
Comment... |
|
Plasmid |
|
Comment... |
