"
Team:Tuebingen/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
| Line 16: | Line 16: | ||
<div id="actualContent"> | <div id="actualContent"> | ||
| - | + | <img src="https://static.igem.org/mediawiki/2013/9/97/Tue_Eppis.jpg" style="float:left; width: 100px"> | |
<h3>General and <i>E. coli</i> protocols</h3> | <h3>General and <i>E. coli</i> protocols</h3> | ||
<ul> | <ul> | ||
Revision as of 20:18, 4 October 2013
Protocols
General and E. coli protocols
- SOB medium
- LB medium
- TAE buffer
- LB plates
- DNA-ladder-buffer mix
- Gelelectrophoresis
- Inoue buffer
- Chemo-competent E. coli cells
- E. coli transformation
- Ligation of digested parts and vectors
- Glycerol stocks
- PCR
- 3A-Assembly
- Preparative restriction digest
- Control restriction digest
- Mini Prep
- Gel Extraction
- Colony-PCR
- Handling of the Tecan reader
Saccharomyces cerevisiae-specific protocols
