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Team:Evry/Chelator
From 2013.igem.org
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<h1>Iron Chelator</h1> | <h1>Iron Chelator</h1> | ||
| - | <p>Here we present Fig 1.a and 1.b our | + | <p>Here we present Fig 1.a and 1.b our constructions Assembly of enterobactin chelator genes entA-entF into a single operon |
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| - | <b>Fig 1.a</b> First | + | <b>Fig 1.a</b> First construction containing the enterobactin synthesis genes Ent A, Ent D and Ent F under the control of Lac O operator. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning. |
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Revision as of 20:41, 4 October 2013
Iron Chelator
Here we present Fig 1.a and 1.b our constructions Assembly of enterobactin chelator genes entA-entF into a single operon
Fig 1.a First construction containing the enterobactin synthesis genes Ent A, Ent D and Ent F under the control of Lac O operator. Genes fusions were made with flanking restriction sites that are compatible with Biobrick-based cloning.
Fig 1.b Second plasmid containing the enterobactin synthesis genes Ent B, Ent C and Ent E.
| NAME | FIGURE | Description |
|---|---|---|
|
Lac promoter |
|
Lac Promoter |
|
RBS + EntA |
|
First gene required for enterobactin sythesis |
|
RBS + EntB |
|
Second gene required for enterobactin sythesis |
|
RBS + EntC |
|
Third gene required for enterobactin sythesis |
|
RBS + EntD |
|
Fourth gene required for enterobactin sythesis |
|
RBS + EntE |
|
Fifth gene required for enterobactin sythesis |
|
RBS + EntF |
|
Sixth gene required for enterobactin synthesis |
|
Terminator |
|
Transcription Stop signal |
|
Plasmid |
|
Backbone with ampicillin resistance |
Table 1. Genetic elements used to produce the enterobactin siderophore.
Future caracterisation of the construction
The first
