Team:USTC CHINA/Notebook/Timeline

From 2013.igem.org

(Difference between revisions)
 
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           <dl>
           <dl>
             <dt>Experiment pet part
             <dt>Experiment pet part
-
             <span>Introduce plasmid containing the GFP sequence into E.coli<br />
+
             <span>Introduce plasmid containing the GFP sequence into <i>E.coli</i><br />
Extract the plasmid after verified by PCR<br />
Extract the plasmid after verified by PCR<br />
Connect GFP gene with TD-1 via PCR<br />
Connect GFP gene with TD-1 via PCR<br />
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Verify the recombined plasmid by PCR<br />
Verify the recombined plasmid by PCR<br />
Sequence the plasmid<br />
Sequence the plasmid<br />
-
Introduce recombined plasmid into E.coli<br />
+
Introduce recombined plasmid into <i>E.coli</i><br />
Verify the bacterium by PCR<br />
Verify the bacterium by PCR<br />
Induce protein expression<br />
Induce protein expression<br />
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           <h3>Apr.15th-Sept.10th<span>2013</span></h3>
           <h3>Apr.15th-Sept.10th<span>2013</span></h3>
           <dl>
           <dl>
-
             <dt>Experiment B.subtilis part
+
             <dt>Experiment <i>B.subtilis</i> part
             <span>Get the GFP sequence via PCR<br />
             <span>Get the GFP sequence via PCR<br />
Connect GFP gene with part of TD-1 via PCR<br />
Connect GFP gene with part of TD-1 via PCR<br />
Line 184: Line 184:
Verify the recombined plasmid by PCR<br />
Verify the recombined plasmid by PCR<br />
Sequence the plasmid<br />
Sequence the plasmid<br />
-
Introduce recombined plasmid into B.subtilis<br />
+
Introduce recombined plasmid into <i>B.subtilis</i><br />
Verify the bacterium by PCR<br />
Verify the bacterium by PCR<br />
Induce protein expression<br />
Induce protein expression<br />

Latest revision as of 12:41, 28 October 2013

2013 USTC_CHINA iGEM Chronology

    2012

  • 2012

  • Dec.15th2012

    Annual recruiting season We brought a large number of inquisitive minds
    to USTC igem team.

    2013

  • Jan.26th2013

    Systematic training begin Senior team members gave systematic training to the fresh
    and assigned responsibilities for every individual.

  • Feb.17th2013

    Second training course We held a simulated iGEM competition.
    Everyone was serious about the task he or she received,
    and gained a lot from the simulated competition.
    In the end, the team leader was elected by us.

  • Mar.2nd2013

    Grouping and brain storming All the members were divided into several groups
    according to their specialty and interest,
    and were motivated in the mobilization meeting.
    Everyone was ready for the coming activities.

  • Mar.30th2013

    Preliminary identified
    several projects Algae produce H2, natural competence
    and magnetosome application
    were preliminary identified as the promising projects.

  • May.15th2013

    SDI Conference Through detailed discussion, we selected
    optimization of blue-green algae produce H2 as our subject.

  • May.31th2013

    Halmatogenesis A recently published paper had already done
    what we prepared to do, and we had to start to
    search another competitve project.

  • June.5th2013

    In situ transdermal vaccine
    born

  • July.10th-Apr.14th2013

    Experiment pet part Introduce plasmid containing the GFP sequence into E.coli
    Extract the plasmid after verified by PCR
    Connect GFP gene with TD-1 via PCR
    Connect the fragment with RBS and locus of restriction
    enzyme digestion via PCR
    Digest the sequence and the plasmid
    with same restriction endonuclease
    Connect the sequence and the plasmid with DNA ligase
    Verify the recombined plasmid by PCR
    Sequence the plasmid
    Introduce recombined plasmid into E.coli
    Verify the bacterium by PCR
    Induce protein expression
    Verify the protein by SDS-page
    Secret protein abundantly
    Concentrate the protein via nickel column
    Verify the protein by SDS-page
    Transdermal experiments

  • Apr.15th-Sept.10th2013

    Experiment B.subtilis part Get the GFP sequence via PCR
    Connect GFP gene with part of TD-1 via PCR
    Connect the fragment with another part of TD-1 via PCR
    Connect the fragment with promoter and
    signal peptide via PCR
    Digest the sequence and the plasmid with
    same restriction endonuclease
    Connect the sequence and the plasmid with DNA ligase
    Verify the recombined plasmid by PCR
    Sequence the plasmid
    Introduce recombined plasmid into B.subtilis
    Verify the bacterium by PCR
    Induce protein expression
    Concentrate the protein via TCA
    Verify the protein by SDS-page
    Secret protein abundantly
    Concentrate by centrifuging
    Verify the protein by SDS-page
    Transdermal experiments

  • Sept.14th2013

    in vivo Transdermal antigen
    antibody response validation
Notebook

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