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Team:NYMU-Taipei/Project/Inhibition/Inhibition circuit
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===Circuit Regulation Testing=== | ===Circuit Regulation Testing=== | ||
<br>1.For pTet (R0040)： | <br>1.For pTet (R0040)： | ||
| - | <p> pTet(R0040)+ E0840....(controll) </p> | + | <p> (1)pTet(R0040)+ E0840....(controll) </p> |
| - | <p> J23102+ RBS+ tetR+ Term+ pTet+ E0840 </p> | + | <p> (2)J23102+ RBS+ tetR+ Term+ pTet+ E0840 </p> |
| - | <p> J23102+ RBS+ lacI+ Term+ pTet+ E0840 </p> | + | <p> (3)J23102+ RBS+ lacI+ Term+ pTet+ E0840 </p> |
<br>2. For pLux/cI hybrid (R0065)： | <br>2. For pLux/cI hybrid (R0065)： | ||
| - | <p> pLux/cI(R0065)+ E0840....(controll) </p> | + | <p> (1)pLux/cI(R0065)+ E0840....(controll) </p> |
| - | <p> J23102+ RBS+ cI+ Term+ R0065+ E0840 </p> | + | <p> (2)J23102+ RBS+ cI+ Term+ R0065+ E0840 </p> |
| - | <p> J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840 </p> | + | <p> (3)J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840 </p> |
| - | <p> J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840</p> | + | <p> (4)J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840</p> |
| + | <br>3. For our killing material concentration estimation: | ||
| + | <p> (1)pLux/cI+ RBS+ E0040+ RBS+ luxI+ RBS+ luxR+ Term </p> | ||
===Our expectation for Testing=== | ===Our expectation for Testing=== | ||
<br>1. | <br>1. | ||
Revision as of 18:49, 28 October 2013
Circuit Regulation Vedio
Circuit Regulation Testing
1.For pTet (R0040)：
(1)pTet(R0040)+ E0840....(controll)
(2)J23102+ RBS+ tetR+ Term+ pTet+ E0840
(3)J23102+ RBS+ lacI+ Term+ pTet+ E0840
2. For pLux/cI hybrid (R0065)：
(1)pLux/cI(R0065)+ E0840....(controll)
(2)J23102+ RBS+ cI+ Term+ R0065+ E0840
(3)J23102+ RBS+ luxR+ RBS+ luxI+ Term+ R0065+ E0840
(4)J23102+ RBS+ luxR+ RBS+ luxI+ RBS+ cI+ Term+ R0065+ E0840
3. For our killing material concentration estimation:
(1)pLux/cI+ RBS+ E0040+ RBS+ luxI+ RBS+ luxR+ Term
Our expectation for Testing
1.
(1)can see GFP under fluroscence
(2)can not see GFP under fluroscence
(3)can see GFP under fluroscence again
Conclusion： tetR coding sequence has the ability to inhibit pTet.</br>
2.
(1)can not see GFP under fluroscence
(2)can not see GFP under fluroscence
(3)can see GFP under fluroscence
(4)can not see GFP under fluroscence
Conclusion： cI coding sequence is more dominant than LuxR/AHL, and cI coding sequence can well inhibit pLux/cI as well as LuxR& LuxI hibrid can initiate pLux/cI only when cI is absent.
