Team:TU-Munich/Results/Overview

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Revision as of 00:27, 29 October 2013

Results Overview

Having spent our summer in the lab, we are proud to present our accomplishments. Upto the European regional jamboree in Lyon, we have created 72 BioBricks and devices, transformed and selected 20 different transgenic GM-mosses and characterized our effectors as recombinant proteins produced in E. coli and in our moss Physcomitrella patens, a chassis newly introduced into iGEM. We took further steps to put our phytoremediation project into practice by developing concepts for the implementation of our PhyscoFilter in the environment and by analyzing the economic potential of this innovative technology in our Entrepreneurship section. Additional to our wetlab work, we contributed a very powerful software tool for the annotation of BioBricks from the Parts Registry and created tutorials to pass this summer´s experiences and skills on to the subsequent iGEM generations.

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BioBricks

We created 72 BioBricks, including BioBricks advancing the use of Physcomitrella patens as a chassis, BioBricks enhancing phytoremediation applications and BioBricks for the light triggered kill-switch mechanism. (Read more)

Effector studies

We selected six different effector proteins and produced them in Escherichia coli and characterized them further concerning stability and activity. (Read more)

Moss Methods

We performed some general experiments to optimize Physcomitrella patens concerning tolerance of toxins, growth optimization and the use of different cultivation surfaces. Furthermore, we created 20 different strains of transformed moss during our visit to Prof. Reski´s lab in Freiburg. (Read more)

Localization

In order to ensure the best functionality for our effectors we designed and used several localization methods aswell as different verification techniques to see if our localizations were efective. (Read more)

PhyscoFilter

After selection and regeneration of the transgenic moss, we started the experiments with them and characterize their properties as PhyscoFilter. (Read more)

Kill Switch

After selection and regeneration of the transgenic moss, we started the experiments with them and characterize their properties as PhyscoFilter. (Read more)

Implementation

The creation of new environmental solutions doesn't stop at the development of GM moss. We tried to find out how a large-scale biofilter could be implemented.
Read more

AutoAnnotator

Protein coding BioBricks constitute large parts of the Parts Registry. We created a software for in-silico characterization of various parameters all summed up in a standardized table. To improve the parts registry, the RFC 96 standard proposes a range of characteristics convenietly determined by the AutoAnnotator. (Read more)

Entrepreneurship

To translate science into applied technology available to the public, economic and business factors play increasingly important roles. We took the first steps into this direction by examining criteria for implementation and possibilities of business models in biotechnology. (Read more)