"
Team:British Columbia/Notebook
From 2013.igem.org
(Difference between revisions)
(→Team Caffeine) |
|||
| Line 27: | Line 27: | ||
==Team Caffeine== | ==Team Caffeine== | ||
| - | ===June 20=== | + | ===June 20, 2013=== |
Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit. | Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit. | ||
| Line 33: | Line 33: | ||
--grace.yi | --grace.yi | ||
| + | |||
| + | ===July 4, 2013=== | ||
Revision as of 01:37, 13 August 2013
iGEM Home
Contents |
Team CRISPR
July 3
List of BioBricks that are/will be on the way:
Cas9:
- Cas9 - Joe and Anna
- pTet -
- const. promoter + Cas9 - Joe
- promoter + Cas9 + Leader + R + spacer + R (on Kan)
- L + R + spacer + R
Target:
- tracerRNA
- repeat + spacer + repeat - Vanins*2 + underlings
- leader - Ray, Fisal, Dan
Decoy:
- PAM + spacer (on Amp)
Team Cinnamaldehyde
4-coumarate-CoA ligase:
- PCR worked
Team Caffeine
June 20, 2013
Joe, Dan, and I made heat-shock competent cells today - they can be found in the top compartment of the -80º Freezer #3 in the room full of freezers (thanks Cam!). We will be ordering primers (through Diane) to modify the TU Munich caffeine biosynthesis BioBricks we got in the Distribution Kit.
We're making a start - what are the other groups up to?
--grace.yi
