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Team:Evry/Notebook/w1
From 2013.igem.org
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<p> The tree following E. coli strains have been prepared to be chemically competent: BL21, DH5α and TOP10.</p> | <p> The tree following E. coli strains have been prepared to be chemically competent: BL21, DH5α and TOP10.</p> | ||
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<p> To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c </p> | <p> To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c </p> | ||
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Revision as of 11:09, 24 July 2013
Week 1: 17th June - 23rd June
Friday, 21st June
The tree following E. coli strains have been prepared to be chemically competent: BL21, DH5α and TOP10.
To check the quality of our work as well as their competence, each strain has been plated on LB medium with ampicillin, kanamycin or chloramphenicol. We also transformed our bacteria with a random plasmid and plated them on LB medium with ampicillin only. We incubated our petri dishes for the week end at 30°c
