Team:Evry/Protocols/02

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<h1 align='center'> Protocol for transformation </h1>
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<h1> Protocol for transformation </h1>
<p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.<br/>
<p>For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.<br/>
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Revision as of 20:08, 9 August 2013

Iron coli project

Protocol for transformation

For 100 µL of chemical competent cells, add 1 µL of plasmidic DNA. IF the ligation protocol is a golden gate, add 5 µL instead.
Let 30 minutes on ice.
Let the cells at 42°C for exactly 50 seconds.
Let 5 minutes on ice.
Resuspend the cells with 1 mL of LB spread on a petri dish then let it at 37°C.

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