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Team:Evry/Notebook/w8
From 2013.igem.org
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<h2>Plasmide 3:</h2> | <h2>Plasmide 3:</h2> | ||
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| - | We started the week by doing the golden gates over again, meaning the GG 3 and GG 2 (2.1 with FurBS 1, 2.2 with FurBS2 | + | We started the week by doing the golden gates over again, meaning the GG 3 and GG 2 (2.1 with FurBS 1, 2.2 with FurBS2 and 2.3 with FurBS3). In fact, our previous control positive had some unwanted white spots (01/08/13), thus suggesting some contaminations during the transformation step. curretnmy waiting |
</p> | </p> | ||
</br> | </br> | ||
<p> | <p> | ||
| - | + | Additionnally, we did the optimization of our PCR over with the temperature gradient for the annealing step. current waiting | |
</p> | </p> | ||
Revision as of 14:01, 5 August 2013
Week 8: 5th August - 11th August
Plasmide 3:
We started the week by doing the golden gates over again, meaning the GG 3 and GG 2 (2.1 with FurBS 1, 2.2 with FurBS2 and 2.3 with FurBS3). In fact, our previous control positive had some unwanted white spots (01/08/13), thus suggesting some contaminations during the transformation step. curretnmy waiting
Additionnally, we did the optimization of our PCR over with the temperature gradient for the annealing step. current waiting
