"
Team:Paris Saclay/Notebook/August/6
From 2013.igem.org
(Difference between revisions)
| Line 75: | Line 75: | ||
We put in culture the 6, 7, 8 samples for every promoter in 5 ml LB + 5 µl Chlorenphenicol (1000x,20µg/ml) | We put in culture the 6, 7, 8 samples for every promoter in 5 ml LB + 5 µl Chlorenphenicol (1000x,20µg/ml) | ||
Incubator over night to 310,15K at 180 RPM | Incubator over night to 310,15K at 180 RPM | ||
| + | |||
| + | ==='''B - PCB sensing system'''=== | ||
| + | |||
| + | ===='''Obtaining the BphA1 promoter'''==== | ||
| + | |||
| + | ====1 - | ||
| + | |||
| + | |||
{{Team:Paris_Saclay/incl_fin}} | {{Team:Paris_Saclay/incl_fin}} | ||
Revision as of 12:35, 11 August 2013
Contents |
Notebook : August 6
Lab work
A - Aerobic/Anaerobic regulation system
Obtaining activator FNR promoter (narK, nirB, narG)
1 - Gel electrophoresis of the colony PCR products ( made the 08/05/2013)on 25 colonies
XiaoJing, Damir, Anaïs
- NarK promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
- NirB promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
- NarG promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
2 - Samples culture
Xiaoing, Anaïs
We put in culture the 6, 7, 8 samples for every promoter in 5 ml LB + 5 µl Chlorenphenicol (1000x,20µg/ml) Incubator over night to 310,15K at 180 RPM
B - PCB sensing system
Obtaining the BphA1 promoter
====1 -
