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Team:Paris Saclay/Notebook/August/6
From 2013.igem.org
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===='''Obtaining the BphA1 promoter'''==== | ===='''Obtaining the BphA1 promoter'''==== | ||
| - | ====1 - Sequence analysis for BB pBphA1 in psB1C3 5 ( clone 4, 17 and 22 ) | + | ====1 - Sequence analysis for BB pBphA1 in psB1C3 5 ( clone 4, 17 and 22 )==== |
IMAGE | IMAGE | ||
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==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''=== | ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''=== | ||
| - | ====1 - PCR product( made the 08/01/2013)digestion to degrade the backbone psB1C3 | + | ====1 - PCR product( made the 08/01/2013)digestion to degrade the backbone psB1C3==== |
Revision as of 13:04, 11 August 2013
Contents |
Notebook : August 6
Lab work
A - Aerobic/Anaerobic regulation system
Obtaining activator FNR promoter (narK, nirB, narG)
1 - Gel electrophoresis of the colony PCR products ( made the 08/05/2013)on 25 colonies
XiaoJing, Damir, Anaïs
- NarK promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
- NirB promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
- NarG promoter in psB1C3
| IMAGE |
|
Expected size : 500 bp
We obtained fragments of the right size. Our backbone contains the Biobrick.
2 - Samples culture
Xiaoing, Anaïs
We put in culture the 6, 7, 8 samples for every promoter in 5 ml LB + 5 µl Chlorenphenicol (1000x,20µg/ml) Incubator over night to 310,15K at 180 RPM
B - PCB sensing system
Obtaining the BphA1 promoter
1 - Sequence analysis for BB pBphA1 in psB1C3 5 ( clone 4, 17 and 22 )
IMAGE
A - Aerobic/Anaerobic regulation system / B - PCB sensing system
1 - PCR product( made the 08/01/2013)digestion to degrade the backbone psB1C3
