Revision as of 17:11, 11 August 2013

Notebook : August 9

Abdou, Anais, Damir, Nadia, XiaoJing

We do the exprience again because the lysis made on wensday didn't happen. It's probably because the phage strain was too old ( 2001)

Picture: lysed cells comparison.

0µl phage(control): the petri dish is cloudy, bacteria are not lysed.
50µl phage: the petri dish is clear, bacteria are lysed by phages.
We used a wild type strain to keep a stock and a Δ fnr E. coli strain to obtain phages that might have encapsidated a Δfnr::Km fragment.

10µl,50µl and 100µl petri dishes are clear so phages are multiplied.

We let the antibiotic over night to select the right strain.

Abdou

Clone 10,14 and 15 plamid extraction using nucleospin kit.

Results: concentration measured by nanodrop

We still have to sequence plasmids in order to verify our results.

August 1st PCR purification to be sure about the experiment. BphR2 part1, BphR2 part2 and RBS_BphR2_part1, FNR part1, FNR part2 and RBS_FNR_part1.

Results:

IMAGE

we have no fragment so we must do again these PCRs

@@ Line 42: / Line 42: @@
 ==='''A - Aerobic/Anaerobic regulation system / B - PCB sensing system'''===
-===='''1 - Gibson assably.'''====
+===='''1 - Gibson assembly.'''====
-August 1st PCR purification to be sure the experiment.
+August 1st PCR purification to be sure about the experiment.
 BphR2 part1, BphR2 part2 and RBS_BphR2_part1, FNR part1, FNR part2 and RBS_FNR_part1.
@@ Line 63: / Line 63: @@
 |}
-we have no fragments so we mu do again these PCRs
+we have no fragment so we must do again these PCRs
 {{Team:Paris_Saclay/incl_fin}}