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Team:TU-Munich/Notebook/Materials
From 2013.igem.org
(→Oligonucleotides) |
(→Oligonucleotides) |
||
| Line 245: | Line 245: | ||
|- | |- | ||
| | | | ||
| + | |SpyTag_fw | ||
| + | |<code>5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |SpyTag_rv |
| + | |<code>5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |PIF3(2-100)_fw | ||
| + | |<code>5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |PIF3(2-100)_rv |
| + | |<code>5'- CCGCTACTAGTATTAACCGG - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |NucA_fw | ||
| + | |<code>5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |NucA_rv |
| + | |<code>5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |AlcR_fw | ||
| + | |<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |AlcR_rv |
| + | |<code>5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |SQ_pAutoRex8_1 | ||
| + | |<code>5'- CGGGGCATCACAGCGTCG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |SQ_pAutoRex8_2 |
| + | |<code>5'- TATCGGTAACCGAGTCCG - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |AlcR_QCI_fw | ||
| + | |<code>5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |AlcR_QCI_rv |
| + | |<code>5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |AlcR_QCII_fw | ||
| + | |<code>5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |AlcR_QCII_rv |
| + | |<code>5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |AlcR_QCIII_fw | ||
| + | |<code>5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |AlcR_QCIII_rv |
| + | |<code>5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |AlcR_QCIV_fw | ||
| + | |<code>5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |AlcR_QCIV_rv |
| + | |<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |pActin_QC_fw | ||
| + | |<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |pActin_QC_rv |
| + | |<code>5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |NPT_QCI_fw | ||
| + | |<code>5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |NPT_QCI_rv |
| + | |<code>5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |NPT_QCII_fw | ||
| + | |<code>5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |NPT_QCII_rv |
| + | |<code>5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |IRES_polio_fw | ||
| + | |<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |IRES_polio_rv |
| + | |<code>5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |BPU_SDMI_r_for | ||
| + | |<code>5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |BPU_SDMI_r_rev |
| + | |<code>5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'</code><br> | ||
|- | |- | ||
| | | | ||
| + | |Strep-TEV_for | ||
| + | |<code>5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'</code><br> | ||
| + | |- | ||
| | | | ||
| - | |<code>5'- | + | |Strep-TEV_rev |
| + | |<code>5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'</code><br> | ||
|} | |} | ||
Revision as of 12:33, 12 August 2013
Reagents
Chemicals
| Reagent | Supplier |
Kits and Standards
Plastic Disposables
Oligonucleotides
| Name | Sequence | |
| EreA_for | 5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘ | |
| EreA-rev | 5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘ | |
| EreA_SDMI_for | 5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘ | |
| EreA_SDMI_rev | 5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3' | |
| EreA_SDMII_for | 5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3' | |
| EreA_SDMII_rev | 5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3' | |
| EreA_SDMIII_for | 5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3' | |
| EreA_SDMIII_rev | 5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3' | |
| EreB_for | 5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3' | |
| EreB-rev | 5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3' | |
| EreB_SDMI_for | 5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3' | |
| EreB_SDMI_rev | 5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3' | |
| EreB_SDMII_for | 5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3' | |
| EreB_SDMII_rev | 5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3' | |
| EreB_SDMIII_for | 5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3' | |
| EreB_SDMIII_rev | 5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3' | |
| TEV_fw | 5'- gcgcgtctagatggccggcggagaaagcttgtttaaggggc - 3' | |
| TEV_rv | 5'- atataaccggtattcatgagttgagtcgcttcc - 3' | |
| TEV_t387c_fw | 5'- atggtgtcagacactagctgcacattcccttcatc - 3' | |
| TEV_t387c_rv | 5'- gatgaagggaatgtgcagctagtgtctgacaccat - 3' | |
| N_split_TEV_fw | 5'- attcgcggccgcttctag - 3' | |
| N_split_TEV_rv | 5'- ttcgaaaccggtagtttggaagttggttgtcac - 3' | |
| C_split_TEV_fw | 5'- aagctttctagatggccggcaagagcatgtctagcatggtg - 3' | |
| C_split_TEV_rv | 5'- ccgctactagtattaaccgg - 3' | |
| PIF6_2-100_fw | 5'- aagctttctagatggccggcatgttcttaccaaccgattattgttgc - 3' | |
| PIF6_2-100_rv | 5'- ttcgaaaccggtgtcaacatgtttattgctttc - 3' | |
| MinMCS_fo | 5'- ctagacctaggaagcttcctgcaggactagtaagcttctgca - 3' | |
| MinMCS_rev | 5'- gaagcttactagtcctgcaggaagcttcctaggt - 3' | |
| Bpu_for | 5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3' | |
| Bpu_rev | 5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3' | |
| Bpu_SDMI_for | 5'- cacttaagcattttctacgggtcgatcacaccattca - 3' | |
| Bpu_SDMI_rev | 5'- tgaatggtgtgatcgacccgtagaaaatgcttaagtg - 3' | |
| Bpu_SDMII_fo | 5'- gacacgattaaatgtgtatgcgggcttagctggattttatttg - 3' | |
| Bpu_SDMII_re | 5'- caaataaaatccagctaagcccgcatacacatttaatcgtgtc - 3' | |
| Bpu_SDMIII_fo | 5'- cacccttaaaaataaagctggctgcggacaggaag - 3' | |
| Bpu_SDMIII_re | 5'- cttcctgtccgcagccagctttatttttaagggtg - 3' | |
| Bpu_SDMIV_fo | 5'- cgtacgctcactcttacgggtacacaggataaata - 3' | |
| Bpu_SDMIV_re | 5'- tatttatcctgtgtacccgtaagagtgagcgtacg - 3' | |
| xylE_for | 5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3' | |
| xylE_rev | 5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3' | |
| xylE_c312a_for | 5'- GAACAGTTGTGGACGGCGCGTGCGC - 3' | |
| xylE_c312a_rev | 5'- GCGCACGCGCCGTCCACAACTGTTC - 3' | |
| xylE_g483a_for | 5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3' | |
| xylE_g483a_rev | 5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3' | |
| xylE_a834g_for | 5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3' | |
| xylE_a834_rev | 5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3' | |
| p Act_for | 5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3' | |
| pAct_rev | 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3' | |
| t35S_for | 5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3' | |
| t35S_rev | 5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3' | |
| Npt_for | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3' | |
| Npt_rev | 5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3' | |
| miniMCSII_fw | 5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3' | |
| miniMCSII_rv | 5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3' | |
| SpyTag_fw | 5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3' | |
| SpyTag_rv | 5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3' | |
| PIF3(2-100)_fw | 5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3' | |
| PIF3(2-100)_rv | 5'- CCGCTACTAGTATTAACCGG - 3' | |
| NucA_fw | 5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3' | |
| NucA_rv | 5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3' | |
| AlcR_fw | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3' | |
| AlcR_rv | 5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3' | |
| SQ_pAutoRex8_1 | 5'- CGGGGCATCACAGCGTCG - 3' | |
| SQ_pAutoRex8_2 | 5'- TATCGGTAACCGAGTCCG - 3' | |
| AlcR_QCI_fw | 5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3' | |
| AlcR_QCI_rv | 5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3' | |
| AlcR_QCII_fw | 5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3' | |
| AlcR_QCII_rv | 5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3' | |
| AlcR_QCIII_fw | 5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3' | |
| AlcR_QCIII_rv | 5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3' | |
| AlcR_QCIV_fw | 5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3' | |
| AlcR_QCIV_rv | 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3' | |
| pActin_QC_fw | 5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3' | |
| pActin_QC_rv | 5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3' | |
| NPT_QCI_fw | 5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3' | |
| NPT_QCI_rv | 5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3' | |
| NPT_QCII_fw | 5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3' | |
| NPT_QCII_rv | 5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3' | |
| IRES_polio_fw | 5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3' | |
| IRES_polio_rv | 5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3' | |
| BPU_SDMI_r_for | 5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3' | |
| BPU_SDMI_r_rev | 5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3' | |
| Strep-TEV_for | 5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3' | |
| Strep-TEV_rev | 5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3' |
Instruments
Electrophoresis
Photometers
Nanodrop Spectrohpotometer
Moss-cultivation
References:
http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984
- http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.
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