Team:TU-Munich/Notebook/Materials

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Reagents

Chemicals

Reagent

Supplier

Kits and Standards

Plastic Disposables

Oligonucleotides

Name	Sequence
EreA_for	`5’- GCGCGTCTAGATGGCCGGCACGTGGAGAACGACCAGA -3‘`
EreA-rev	`5‘- TTCGAAACCGGTTAGGGCAACTAGGCTGTCC -3‘`
EreA_SDMI_for	`5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘`
EreA_SDMI_rev	`5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'`
EreA_SDMII_for	`5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'`
EreA_SDMII_rev	`5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'`
EreA_SDMIII_for	`5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'`
EreA_SDMIII_rev	`5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'`
EreB_for	`5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'`
EreB-rev	`5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'`
EreB_SDMI_for	`5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'`
EreB_SDMI_rev	`5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'`
EreB_SDMII_for	`5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'`
EreB_SDMII_rev	`5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'`
EreB_SDMIII_for	`5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'`
EreB_SDMIII_rev	`5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'`
TEV_fw	`5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3'`
TEV_rv	`5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3'`
TEV_t387c_fw	`5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3'`
TEV_t387c_rv	`5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3'`
N_split_TEV_fw	`5'- ATTCGCGGCCGCTTCTAG - 3'`
N_split_TEV_rv	`5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3'`
C_split_TEV_fw	`5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3'`
C_split_TEV_rv	`5'- CCGCTACTAGTATTAACCGG - 3'`
PIF6_2-100_fw	`5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3'`
PIF6_2-100_rv	`5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3'`
MinMCS_fo	`5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'`
MinMCS_rev	`5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3'`
Bpu_for	`5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'`
Bpu_rev	`5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'`
Bpu_SDMI_for	`5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3'`
Bpu_SDMI_rev	`5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3'`
Bpu_SDMII_fo	`5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3'`
Bpu_SDMII_re	`5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3'`
Bpu_SDMIII_fo	`5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3'`
Bpu_SDMIII_re	`5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3'`
Bpu_SDMIV_fo	`5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3'`
Bpu_SDMIV_re	`5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3'`
xylE_for	`5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG - 3'`
xylE_rev	`5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC - 3'`
xylE_c312a_for	`5'- GAACAGTTGTGGACGGCGCGTGCGC - 3'`
xylE_c312a_rev	`5'- GCGCACGCGCCGTCCACAACTGTTC - 3'`
xylE_g483a_for	`5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'`
xylE_g483a_rev	`5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'`
xylE_a834g_for	`5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'`
xylE_a834_rev	`5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'`
p Act_for	`5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'`
pAct_rev	`5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'`
t35S_for	`5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'`
t35S_rev	`5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'`
Npt_for	`5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'`
Npt_rev	`5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'`
miniMCSII_fw	`5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'`
miniMCSII_rv	`5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'`
SpyTag_fw	`5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'`
SpyTag_rv	`5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'`
PIF3(2-100)_fw	`5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'`
PIF3(2-100)_rv	`5'- CCGCTACTAGTATTAACCGG - 3'`
NucA_fw	`5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'`
NucA_rv	`5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'`
AlcR_fw	`5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'`
AlcR_rv	`5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'`
SQ_pAutoRex8_1	`5'- CGGGGCATCACAGCGTCG - 3'`
SQ_pAutoRex8_2	`5'- TATCGGTAACCGAGTCCG - 3'`
AlcR_QCI_fw	`5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'`
AlcR_QCI_rv	`5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'`
AlcR_QCII_fw	`5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'`
AlcR_QCII_rv	`5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'`
AlcR_QCIII_fw	`5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'`
AlcR_QCIII_rv	`5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'`
	AlcR_QCIV_fw	`5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'`
AlcR_QCIV_rv	`5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'`
pActin_QC_fw	`5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'`
pActin_QC_rv	`5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'`
NPT_QCI_fw	`5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'`
NPT_QCI_rv	`5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'`
NPT_QCII_fw	`5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'`
NPT_QCII_rv	`5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'`
IRES_polio_fw	`5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'`
IRES_polio_rv	`5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'`
BPU_SDMI_r_for	`5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'`
BPU_SDMI_r_rev	`5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'`
Strep-TEV_for	`5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'`
Strep-TEV_rev	`5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'`
SQ_pluc-Actin_1	`5'- CCGACTCAAATACAGATATGC - 3'`
SQ_pluc-Actin_2	`5'- CTCGGATGTAGATCTGCG - 3'`
pActin_QC_ins_fw	`5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC ACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'`
pActin_QC_ins_rv	`5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT GGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'`
pActin_Oryza_fw	`5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'`
pActin_Oryza_rv	`5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'`
GGGGSx5_TEV_fw	`5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG CGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'`
GGGGSx5_TEV_rv	`5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC GATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'`
mMCSII_fw	`5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'`
mMCSII_rv	`5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'`
FluA_tripmut_fw	`5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'`
FluA_tripmut_rv	`5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'`
pActin_QCI_fw	`5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'`
pActin_QCI_rv	`5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'`
Alc R fw Seq.	`5'- CAGAGCACAATTGTCCATAC - 3'`
Alc R rv Seq.	`5'- TCGCGGTCGATGCTCTCC - 3'`
pActin_QCins_fw	`5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC CACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'`
pActin_QCins_rv	`5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG TTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'`
PP1_for	`5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'`
PP1_rev	`5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'`
PhyB fw Seq	`5'- GCGATTTCTCAGTTACAGGC - 3'`
PhyB rv Seq.	`5'- CTCAAAGCACGAGAAAGAAGC - 3'`
pAct_ins_fw	`5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3'`
pAct_ins_rv	`5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3'`
PppAct_fw	`5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3'`
PppAct_rv	`5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3'`
PppActin_QC1_fw	`5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3'`
PppActin_QC1_rv	`5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3'`
PppActin_QC2_fw	`5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3'`
PppActin_QC2_rv	`5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3'`
PppActin_fw_corr	`5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3'`
SYFP2_fw	`5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3'`
SYFP2_rv	`5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3'`
eCFP_fw	`5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3'`
eCFP_rv	`5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3'`
G-TEV-site-G_fw	`5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'`
G-TEV-site-G_fw	`5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT - 3'`
PppActin_seq_fw	`5'- CATGTCCCAGGGCCCTAG - 3'`

Instruments

Electrophoresis

Photometers

Nanodrop Spectrohpotometer

Moss-cultivation

References:

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984

http://www.ncbi.nlm.nih.gov/pubmed/6327079 Edens et al., 1984 Edens, L., Bom, I., Ledeboer, A. M., Maat, J., Toonen, M. Y., Visser, C., and Verrips, C. T. (1984). Synthesis and processing of the plant protein thaumatin in yeast. Cell, 37(2):629–33.

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@@ Line 36: / Line 36: @@
 |<code>5’- CGAATCAGGAAGAAAACTACAGTTAGTCGGAATCGAC -3‘</code><br>
 |-
-|
 |EreA_SDMI_rev
 |<code>5'- GTCGATTCCGACTAACTGTAGTTTTCTTCCTGATTCG -3'</code><br>
 |-
-|
 |EreA_SDMII_for
 |<code>5'- GACGCCTGTGGTACGGAAAATTCATGTCTGACATT - 3'</code><br>
 |-
-|
 |EreA_SDMII_rev
 |<code>5'- AATGTCAGACATGAATTTTCCGTACCACAGGCGTC - 3'</code><br>
 |-
-|
 |EreA_SDMIII_for
 |<code>5'- GTGTTCCAAGCGCGGGCAAGGACAGCC - 3'</code><br>
 |-
-|
 |EreA_SDMIII_rev
 |<code>5'- GGCTGTCCTTGCCCGCGCTTGGAACAC - 3'</code><br>
 |-
-|
 | EreB_for
 |<code>5'- GCGCGTCTAGATGGCCGGCAGGTTCGAAGAATGGGTC - 3'</code><br>
 |-
-|
 | EreB-rev
 |<code>5'- TTCGAAACCGGTTTCATAAACGACCTCAGATACAG - 3'</code><br>
 |-
-|
 |EreB_SDMI_for
 |<code>5'- GGTGAAAATTCTCATTTCATAAAAGAGTTCTTTTTGTTACGACATACGCTTTT - 3'</code><br>
 |-
-|
 |EreB_SDMI_rev
 |<code>5'- AAAAGCGTATGTCGTAACAAAAAGAACTCTTTTATGAAATGAGAATTTTCACC - 3'</code><br>
 |-
-|
 |EreB_SDMII_for
 |<code>5'- CTTATAGTGGGCATACAGCAGCCCTCTATCCGG - 3'</code><br>
 |-
-|
 | EreB_SDMII_rev
 |<code>5'- CCGGATAGAGGGCTGCTGTATGCCCACTATAAG - 3'</code><br>
 |-
-|
 |EreB_SDMIII_for
 |<code>5'- CGAGTTGATAACTTCCAACTACAGGAACCAAATGAAGGTTC - 3'</code><br>
 |-
-|
 |EreB_SDMIII_rev
 |<code>5'- GAACCTTCATTTGGTTCCTGTAGTTGGAAGTTATCAACTCG - 3'</code><br>
 |-
-|
 |TEV_fw
 |<code>5'- GCGCGTCTAGATGGCCGGCGGAGAAAGCTTGTTTAAGGGGC - 3'</code><br>
 |-
-|
 |TEV_rv
 |<code>5'- ATATAACCGGTATTCATGAGTTGAGTCGCTTCC - 3'</code><br>
 |-
-|
 |TEV_t387c_fw
 |<code>5'- ATGGTGTCAGACACTAGCTGCACATTCCCTTCATC - 3'</code><br>
 |-
-|
 |TEV_t387c_rv
 |<code>5'- GATGAAGGGAATGTGCAGCTAGTGTCTGACACCAT - 3'</code><br>
 |-
-|
 |N_split_TEV_fw
 |<code>5'- ATTCGCGGCCGCTTCTAG - 3'</code><br>
 |-
-|
 |N_split_TEV_rv
 |<code>5'- TTCGAAACCGGTAGTTTGGAAGTTGGTTGTCAC - 3'</code><br>
 |-
-|
 |C_split_TEV_fw
 |<code>5'- AAGCTTTCTAGATGGCCGGCAAGAGCATGTCTAGCATGGTG - 3'</code><br>
 |-
-|
 |C_split_TEV_rv
 |<code>5'- CCGCTACTAGTATTAACCGG - 3'</code><br>
 |-
-|
 |PIF6_2-100_fw
 |<code>5'- AAGCTTTCTAGATGGCCGGCATGTTCTTACCAACCGATTATTGTTGC - 3'</code><br>
 |-
-|
 |PIF6_2-100_rv
 |<code>5'- TTCGAAACCGGTGTCAACATGTTTATTGCTTTC - 3'</code><br>
 |-
-|
 |MinMCS_fo
 |<code>5'- CTAGACCTAGGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'</code><br>
 |-
-|
 |MinMCS_rev
 |<code>5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCCTAGGT - 3'</code><br>
 |-
-|
 |Bpu_for
 |<code>5'- GCGCGTCTAGATGGCCGGCAACCTAGAAAAATTTGTTGACGAGCTG - 3'</code><br>
 |-
-|
 |Bpu_rev
 |<code>5'- TTCGAAACCGGTGATGATATCCATCGGCCGCAT - 3'</code><br>
 |-
-|
 |Bpu_SDMI_for
 |<code>5'- CACTTAAGCATTTTCTACGGGTCGATCACACCATTCA - 3'</code><br>
 |-
-|
 |Bpu_SDMI_rev
 |<code>5'- TGAATGGTGTGATCGACCCGTAGAAAATGCTTAAGTG - 3'</code><br>
 |-
-|
 |Bpu_SDMII_fo
 |<code>5'- GACACGATTAAATGTGTATGCGGGCTTAGCTGGATTTTATTTG - 3'</code><br>
 |-
-|
 |Bpu_SDMII_re
 |<code>5'- CAAATAAAATCCAGCTAAGCCCGCATACACATTTAATCGTGTC - 3'</code><br>
 |-
-|
 |Bpu_SDMIII_fo
 |<code>5'- CACCCTTAAAAATAAAGCTGGCTGCGGACAGGAAG - 3'</code><br>
 |-
-|
 |Bpu_SDMIII_re
 |<code>5'- TTCCTGTCCGCAGCCAGCTTTATTTTTAAGGGTG - 3'</code><br>
 |-
-|
 |Bpu_SDMIV_fo
 |<code>5'- CGTACGCTCACTCTTACGGGTACACAGGATAAATA - 3'</code><br>
 |-
-|
 |Bpu_SDMIV_re
 |<code>5'- TATTTATCCTGTGTACCCGTAAGAGTGAGCGTACG - 3'</code><br>
 |-
-|
 |xylE_for
 |<code>5'- AAGCTTTCTAGATGGCCGGCAACAAAGGTGTAATGCGACCG  - 3'</code><br>
 |-
-|
 |xylE_rev
 |<code>5'- TTCGAAACCGGTGGTCAGCACGGTCATGAATC  - 3'</code><br>
 |-
-|
 |xylE_c312a_for
 |<code>5'- GAACAGTTGTGGACGGCGCGTGCGC  - 3'</code><br>
 |-
-|
 |xylE_c312a_rev
 |<code>5'- GCGCACGCGCCGTCCACAACTGTTC - 3'</code><br>
 |-
-|
 |xylE_g483a_for
 |<code>5'- CCTCATGTATGGCGACGAATTACCGGCGACCTA - 3'</code><br>
 |-
-|
 |xylE_g483a_rev
 |<code>5'- TAGGTCGCCGGTAATTCGTCGCCATACATGAGG - 3'</code><br>
 |-
-|
 |xylE_a834g_for
 |<code>5'- CAACTACCCGGACCACAAGCCGGTGACCT - 3'</code><br>
 |-
-|
 |xylE_a834_rev
 |<code>5'- AGGTCACCGGCTTGTGGTCCGGGTAGTTG - 3'</code><br>
 |-
-|
 |p Act_for
 |<code>5'- AAGCTTTCTAGAGTCGACGAGGTCATTCATATG - 3'</code><br>
 |-
-|
 |pAct_rev
 |<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGCTTCTACCTACAAAAAAGCTC - 3'</code><br>
 |-
-|
 |t35S_for
 |<code>5'- AAGCTTTCTAGAGCTAGAGTCCGCAAAAATCACC - 3'</code><br>
 |-
-|
 |t35S_rev
 |<code>5'- CTCGAGCTGCAGCGGCCGCTACTAGTAGGTCACTGGATTTTGGTTTTAGG - 3'</code><br>
 |-
-|
 |Npt_for
 |<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGATCGGATCCTGTCAAACAC - 3'</code><br>
 |-
-|
 |Npt_rev
 |<code>5'- CGGCCGCTACTAGTAATCAAGCTTGACAGGAGGC - 3'</code><br>
 |-
-|
 |miniMCSII_fw
 |<code>5'- CTAGACAATTGAAGCTTCCTGCAGGACTAGTAAGCTTCTGCA - 3'</code><br>
 |-
-|
 |miniMCSII_rv
 |<code>5'- GAAGCTTACTAGTCCTGCAGGAAGCTTCAATTGT - 3'</code><br>
 |-
-|
 |SpyTag_fw
 |<code>5'- CTAGATGGCCGGCGCTCATATTGTCATGGTTGATGCTTACAAGCCAACTAAGA - 3'</code><br>
 |-
-|
 |SpyTag_rv
 |<code>5'- CCGGTCTTAGTTGGCTTGTAAGCATCAACCATGACAATATGAGCGCCGGCCAT - 3'</code><br>
 |-
-|
 |PIF3(2-100)_fw
 |<code>5'- AAGCTTTCTAGATGGCCGGCCCTCTGTTTGAACTTTTCAGG - 3'</code><br>
 |-
-|
 |PIF3(2-100)_rv
 |<code>5'- CCGCTACTAGTATTAACCGG - 3'</code><br>
 |-
-|
 |NucA_fw
 |<code>5'- AAGCTTTCTAGATGGCCGGCGCAACTTCAACTAAAAAATTACATAAAGAACCTG - 3'</code><br>
 |-
-|
 |NucA_rv
 |<code>5'- TTCGAAACCGGTTTGACCTGAATCAGCGTTGTC - 3'</code><br>
 |-
-|
 |AlcR_fw
 |<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGATGGCAGATACGCGCCGAC - 3'</code><br>
 |-
-|
 |AlcR_rv
 |<code>5'- TTCGAAACTAGTACTACAAAAAGCTGTCAACTTTCCCATTCAAACC - 3'</code><br>
 |-
-|
 |SQ_pAutoRex8_1
 |<code>5'- CGGGGCATCACAGCGTCG - 3'</code><br>
 |-
-|
 |SQ_pAutoRex8_2
 |<code>5'- TATCGGTAACCGAGTCCG - 3'</code><br>
 |-
-|
 |AlcR_QCI_fw
 |<code>5'- GGCTAAATGTTCCTGCTGACATAGCCGCCGATG - 3'</code><br>
 |-
-|
 |AlcR_QCI_rv
 |<code>5'- CATCGGCGGCTATGTCAGCAGGAACATTTAGCC - 3'</code><br>
 |-
-|
 |AlcR_QCII_fw
 |<code>5'- GCACGGTATGGGTGCACGTTTGGACAAGCTACTCGAAAATG - 3'</code><br>
 |-
-|
 |AlcR_QCII_rv
 |<code>5'- CATTTTCGAGTAGCTTGTCCAAACGTGCACCCATACCGTGC - 3'</code><br>
 |-
-|
 |AlcR_QCIII_fw
 |<code>5'- CATCCAGTCTTGGTACGTCATTTTGGACGGTCACTGGCAT - 3'</code><br>
 |-
-|
 |AlcR_QCIII_rv
 |<code>5'- ATGCCAGTGACCGTCCAAAATGACGTACCAAGACTGGATG - 3'</code><br>
@@ Line 308: / Line 241: @@
 |<code>5'- GCCGCAGCTGCAAGTACCCCGCTAG - 3'</code><br>
 |-
-|
 |AlcR_QCIV_rv
 |<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br>
 |-
-|
 |pActin_QC_fw
 |<code>5'- ATTTTTGAGAAAAATATATATTCAGGCGAATTGCACAATGAACAATAATAAGATTAAAATAG - 3'</code><br>
 |-
-|
 |pActin_QC_rv
 |<code>5'- CTATTTTAATCTTATTATTGTTCATTGTGCAATTCGCCTGAATATATATTTTTCTCAAAAAT - 3'</code><br>
 |-
-|
 |NPT_QCI_fw
 |<code>5'- GTCCGGTGCCCTGAATGAATTGCAGGACGAG - 3'</code><br>
 |-
-|
 |NPT_QCI_rv
 |<code>5'- CTCGTCCTGCAATTCATTCAGGGCACCGGAC - 3'</code><br>
 |-
-|
 |NPT_QCII_fw
 |<code>5'- GCGGGACCCAAGCTTTAGATCTTGCTGCG - 3'</code><br>
 |-
-|
 |NPT_QCII_rv
 |<code>5'- CGCAGCAAGATCTAAAGCTTGGGTCCCGC - 3'</code><br>
 |-
-|
 |IRES_polio_fw
 |<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGTTAAAACAGCTCTGGGGTTG - 3'</code><br>
 |-
-|
 |IRES_polio_rv
 |<code>5'- TTGCAACTGCAGCGGCCGCTACTAGTAAATCCAATTCGCTTTATGATAACAATC - 3'</code><br>
 |-
-|
 |BPU_SDMI_r_for
 |<code>5'- CCACTTAAGCATTTTCTACCAGTCGATCACACCATTCACG - 3'</code><br>
 |-
-|
 |BPU_SDMI_r_rev
 |<code>5'- CGTGAATGGTGTGATCGACTGGTAGAAAATGCTTAAGTGG - 3'</code><br>
 |-
-|
 |Strep-TEV_for
 |<code>5'- CTAGATGGCCGGCAGCGCTTGGTCTCATCCTCAATTTGAAAAGGGTGAAAATCTTTATTTTCAAAGCGGAA - 3'</code><br>
 |-
-|
 |Strep-TEV_rev
 |<code>5'- CCGGTTCCGCTTTGAAAATAAAGATTTTCACCCTTTTCAAATTGAGGATGAGACCAAGCGCTGCCGGCCAT - 3'</code><br>
 |-
-|
 |SQ_pluc-Actin_1
 |<code>5'- CCGACTCAAATACAGATATGC - 3'</code><br>
 |-
-|
 |SQ_pluc-Actin_2
 |<code>5'- CTCGGATGTAGATCTGCG - 3'</code><br>
 |-
-|
 |pActin_QC_ins_fw
 |<code>5'- ACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACC<br>ACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACG - 3'</code><br>
 |-
-|
 |pActin_QC_ins_rv
 |<code>5'- CGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGT<br>GGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGT - 3'</code><br>
 |-
-|
 |pActin_Oryza_fw
 |<code>5'- AAGCTTTCTAGAGTAGCTAGCATACTCGAGGTCATTCATATGCTTG - 3'</code><br>
 |-
-|
 |pActin_Oryza_rv
 |<code>5'- TTCGAACTGCAGCGGCCGCTACTAGTACTTCTACCTACAAAAAAGCTCCG - 3'</code><br>
 |-
-|
 |GGGGSx5_TEV_fw
 |<code>5'- CTAGATGGCCGGCGGTGGAGGCGGTTCAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGGGAGG<br>CGGTGGCTCCGGAGGTGGAGGCTCAGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'</code><br>
 |-
-|
 |GGGGSx5_TEV_rv
 |<code>5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCTGAGCCTCCACCTCCGGAGCCACCGCCTCCC<br>GATCCGCCACCGCCAGAGCCACCTCCGCCTGAACCGCCTCCACCGCCGGCCAT - 3'</code><br>
 |-
-|
 |mMCSII_fw
 |<code>5'- AATTCGCGGCCGCTTCTAGAGCAATTGAAGCTTCCTGCAGGTA - 3'</code><br>
 |-
-|
 |mMCSII_rv
 |<code>5'- CTAGTACCTGCAGGAAGCTTCAATTGCTCTAGAAGCGGCCGCG - 3'</code><br>
 |-
-|
 |FluA_tripmut_fw
 |<code>5'- AAGCTTTCTAGATGGCCGGCGACGTGTACCACGACGGTG - 3'</code><br>
 |-
-|
 |FluA_tripmut_rv
 |<code>5'- TTCGAAACCGGTATTGTTGACCTTGCAGGCGG - 3'</code><br>
 |-
-|
 |pActin_QCI_fw
 |<code>5'- CATAATTTTTGAGAAAAATATATATTCAGGCGAATTGTCACAATGAACAATAATAAGATTAAAATA - 3'</code><br>
 |-
-|
 |pActin_QCI_rv
 |<code>5'- TATTTTAATCTTATTATTGTTCATTGTGACAATTCGCCTGAATATATATTTTTCTCAAAAATTATG - 3'</code><br>
 |-
-|
 |Alc R fw Seq.
 |<code>5'- CAGAGCACAATTGTCCATAC - 3'</code><br>
 |-
-|
 |Alc R rv Seq.
 |<code>5'- TCGCGGTCGATGCTCTCC - 3'</code><br>
 |-
-|
 |pActin_QCins_fw
 |<code>5'- CATCGCCACTATATACATACCCCCCCCTCTCCTCCCATCCCCCCAACCCTACCACCACCACCAC<br>CACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTCCTC - 3'</code><br>
 |-
-|
 |pActin_QCins_rv
 |<code>5'- GAGGAGCTCGTCGTCCGGCAGCGAGGGGGGAGGAGGTGGAGGTGGTGGTGGTGGTGGTGGTAGGG<br>TTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATG - 3'</code><br>
 |-
-|
 |PP1_for
 |<code>5'- GCCGCTTCTAGATGGCCGGCGGATCCGCGGATTTAGATA - 3'</code><br>
 |-
-|
 |PP1_rev
 |<code>5'- GTATTAACCGGTTTTCTTTGCTTGCTTTGTGATC - 3'</code><br>
 |-
-|
 |PhyB fw Seq
 |<code>5'- GCGATTTCTCAGTTACAGGC - 3'</code><br>
 |-
-|
 |PhyB rv Seq.
 |<code>5'- CTCAAAGCACGAGAAAGAAGC - 3'</code><br>
 |-
-|
 |pAct_ins_fw
 |<code>5'- ACCACCACCACCTCCACCTCCTCCCCCCTCGCTGCCGGACGACGAGCTC - 3'</code><br>
 |-
-|
 |pAct_ins_rv
 |<code>5'- GGTGGTGGTAGGGTTGGGGGGATGGGAGGAGAGGGGGGGGTATGTATATAGTGGCGATGG - 3'</code><br>
 |-
-|
 |PppAct_fw
 |<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGATCGAGTCCTTGTGACTTTTGTGCAT - 3'</code><br>
 |-
-|
 |PppAct_rv
 |<code>5'- TTCGAAACTAGTATATTTAATACGGACCTGCACAACAACCACCAAT - 3'</code><br>
 |-
-|
 |PppActin_QC1_fw
 |<code>5'- TTATTGAGGATATCCATCTATCTTGATTTTGGACAATGTTTTACTGC - 3'</code><br>
 |-
-|
 |PppActin_QC1_rv
 |<code>5'- GCAGTAAAACATTGTCCAAAATCAAGATAGATGGATATCCTCAATAA - 3'</code><br>
 |-
-|
 |PppActin_QC2_fw
 |<code>5'- ACACTCTACATTCATGTCAAGAGTATAGGGACTTGGG - 3'</code><br>
 |-
-|
 |PppActin_QC2_rv
 |<code>5'- CCCAAGTCCCTATACTCTTGACATGAATGTAGAGTGT - 3'</code><br>
 |-
-|
 |PppActin_fw_corr
 |<code>5'- AAGCTTGAATTCGCGGCCGCTTCTAGAGCGAGTCCTTGTGACTTTTGTGCAT - 3'</code><br>
 |-
-|
 |SYFP2_fw
 |<code>5'- AAGCTTTCTAGATGGCCGGCGTTAGCAAGGGCGAAGAAC - 3'</code><br>
 |-
-|
 |SYFP2_rv
 |<code>5'- TTCGAAACCGGTTTTATACAGCTCATCCATACCCAG - 3'</code><br>
 |-
-|
 |eCFP_fw
 |<code>5'- AAGCTTTCTAGATGGCCGGCGTGAGCAAGGGCGAGGAG - 3'</code><br>
 |-
-|
 |eCFP_rv
 |<code>5'- TTCGAAACCGGTCTTGTACAGCTCGTCCATGCC - 3'</code><br>
 |-
-|
 |G-TEV-site-G_fw
 |<code>5'- CTAGATGGCCGGCGGAGAGAATTTGTATTTTCAGTCAGGAA - 3'</code><br>
 |-
-|
 |G-TEV-site-G_fw
 |<code>5'- CCGGTTCCTGACTGAAAATACAAATTCTCTCCGCCGGCCAT  - 3'</code><br>
 |-
-|
 |PppActin_seq_fw
 |<code>5'- CATGTCCCAGGGCCCTAG - 3'</code><br>