Team:Evry/Notebook/w9

From 2013.igem.org

(Difference between revisions)
Line 16: Line 16:
Preculture with M9 medium (with carbenicillin) have been launched for BL21 transformed with our 1<sup>st</sup> construction:
Preculture with M9 medium (with carbenicillin) have been launched for BL21 transformed with our 1<sup>st</sup> construction:
</p>
</p>
 +
<ul>
<ul>
<li>Natural Fur Binding Site of Fec A + sfGFP (clone 1, 2, 3)
<li>Natural Fur Binding Site of Fec A + sfGFP (clone 1, 2, 3)
Line 22: Line 23:
</ul>
</ul>
<p>
<p>
 +
<b>Notes:</b> Preculture have been made in M9 medium with iron in oder to inhibit the expression of sfGFP.
 +
</p>
 +
<p>
 +
After one night of culture, time the precultures have been refreshed by diluting them 200 times in M9 medium (with iron and carbenicillin).
</p>
</p>
 +
 +
<h2>2<sup>nd</sup> construction</h2>
 +
 +
<h3>Sequencing preparation</h3>
 +
 +
<p>
 +
Preculture with LB medium (with carbenicillin) have been launched for Top10 transformed with our 2<sup>nd</sup> construction, then minipreps have been realized using the miniprep kit from Machrey Nagel.
 +
</p>
 +
 +
<table>
 +
<thead>
 +
  <tr>
 +
  <th>
 +
    Name
 +
  </th>
 +
  <th>
 +
    Clone
 +
  </th>
 +
  <th>
 +
    Concentration<br/>
 +
    (ng/µL)
 +
  </th>
 +
  <th>
 +
    260/280
 +
  </th>
 +
  <th>
 +
    260/230
 +
  </th>
 +
  </tr>
 +
</thead>
 +
<tbody>
 +
  <tr>
 +
  <td>
 +
   
 +
  </td>
 +
  <td>
 +
   
 +
  </td>
 +
  </tr>
 +
</tbody>
 +
</table>
</div>
</div>

Revision as of 11:09, 13 August 2013

Iron coli project

Week 9: 12th August - 18th August

TECAN

Using the TECAN analysis, we are measuring the capacity of repression of the natural binding site that we extract from the E.coli's genome.

Preculture with M9 medium (with carbenicillin) have been launched for BL21 transformed with our 1st construction:

  • Natural Fur Binding Site of Fec A + sfGFP (clone 1, 2, 3)
  • Natural Fur Binding Site of Fep A + sfGFP (clone 1, 2, 3)
  • Natural Fur Binding Site of Ace B + sfGFP (clone 1, 2, 3)

Notes: Preculture have been made in M9 medium with iron in oder to inhibit the expression of sfGFP.

After one night of culture, time the precultures have been refreshed by diluting them 200 times in M9 medium (with iron and carbenicillin).

2nd construction

Sequencing preparation

Preculture with LB medium (with carbenicillin) have been launched for Top10 transformed with our 2nd construction, then minipreps have been realized using the miniprep kit from Machrey Nagel.

Name Clone Concentration
(ng/µL) 		260/280 260/230

Retrieved from "http://2013.igem.org/Team:Evry/Notebook/w9"