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Timer Plus Sumo
From 2013.igem.org
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<center> | <center> | ||
<img src="https://static.igem.org/mediawiki/2013/9/96/Timer%2Bsumo.png" > | <img src="https://static.igem.org/mediawiki/2013/9/96/Timer%2Bsumo.png" > | ||
| - | <p>Figure 1: Circuit of the timer | + | <p>Figure 1: Circuit of the timer including sumo cleaving</p></div> |
</center> | </center> | ||
</br></br></br></br></br></br></br></br></br></br> | </br></br></br></br></br></br></br></br></br></br> | ||
| - | |||
<h2 align="center">Differential Equations</h2> | <h2 align="center">Differential Equations</h2> | ||
<p align="justify"> | <p align="justify"> | ||
| - | The above circuit can be represented by the following | + | The above circuit can be represented by the following differential equations. We assume a binary |
behavior of the T7 promoter. In the presence of IPTG, the T7 promoter will be active. So, we make | behavior of the T7 promoter. In the presence of IPTG, the T7 promoter will be active. So, we make | ||
the assumption that the T7 is binary variable with two possible states: either active 1 or inactive 0, | the assumption that the T7 is binary variable with two possible states: either active 1 or inactive 0, | ||
Revision as of 14:16, 15 August 2013
Timer Plus Sumo
In this section the system of Figure 1 is modeled. The structure of the timer is very similar version of the timer compared to the construct of iGEM TU Delft team 2009. Here the input is changed to a T7 promoter and the output to Ulp-1. Furthermore, the Ulp-1 cleaves off the SUMO from the peptide combined with the SUMO.
Figure 1: Circuit of the timer including sumo cleaving
Differential Equations
The above circuit can be represented by the following differential equations. We assume a binary behavior of the T7 promoter. In the presence of IPTG, the T7 promoter will be active. So, we make the assumption that the T7 is binary variable with two possible states: either active 1 or inactive 0, this is the variable s.
