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KillSwitch
From 2013.igem.org
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<img src="https://static.igem.org/mediawiki/2013/5/5b/Kill_switch.png" width="600" > | <img src="https://static.igem.org/mediawiki/2013/5/5b/Kill_switch.png" width="600" > | ||
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| - | + | <h2 align="center">Parameters</h2> | |
| - | + | <p align="justify">The used parameters are listed in Table 1. The dimer binding | |
| - | + | strengths are fi�tted on literature of Holin expression using the PcI promoter with and without antiholin | |
| - | + | [14]. | |
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Revision as of 11:17, 16 August 2013
Kill Switch
The kill switch design is based on the expression of holin and antiholin, Figure 1. Holin is a protein that forms pores in cell membranes. Anti-holin binds to holin and inhibits it's action. Once pores are formed by holin, lysozyme can access the periplasmic space and degrade the cell wall, causing cell lysis.
Kill switch constitutes a crucial part of our final system. It is activated after the production and release of the antimicrobial peptide. In that way, E.coli is killed and the safety of the system is ensured.
Figure 1: Circuit of the kill switch
Differential Equations
The kill switch circuit can be represented by the following differential equations.
Parameters
The used parameters are listed in Table 1. The dimer binding strengths are fi�tted on literature of Holin expression using the PcI promoter with and without antiholin [14].
