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Team:Paris Saclay/Notebook/August/14
From 2013.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
===='''Objective : obtaining ...'''==== | ===='''Objective : obtaining ...'''==== | ||
| - | =====1 - | + | =====1 - Electrophoresis to check the digestion of Bba_K1155004,Bba_K1155005, Bba_K1155006 by EcoRI and SpeI===== |
Anaïs, Nadia | Anaïs, Nadia | ||
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We obtained fragments of the right size but in very few quantity. We do it again but this time we will use more quantity of enzymes AND ADN ?! | We obtained fragments of the right size but in very few quantity. We do it again but this time we will use more quantity of enzymes AND ADN ?! | ||
| - | =====2 - Digestion of Bba_K1155004,Bba_K1155005, Bba_K1155006 by SpeI and EcoRI/SpeI===== | + | =====2 - Digestion of Bba_K1155000, Bba_K1155004,Bba_K1155005, Bba_K1155006 by SpeI and EcoRI/SpeI===== |
Anaïs, Nadia | Anaïs, Nadia | ||
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**ADN : 20µL | **ADN : 20µL | ||
**H20 : 3µL | **H20 : 3µL | ||
| + | |||
| + | ====3 - Electrophoresis to check the digestion of Bba_K1155000, Bba_K1155004,Bba_K1155005, Bba_K1155006 by SpeI and EcoRI/SpeI===== | ||
| + | Anaïs, Nadia | ||
| + | |||
| + | {| | ||
| + | | style="width:350px;border:1px solid black;" | [[]] | ||
| + | | style="width:350px;border:1px solid black;vertical-align:top;" | | ||
| + | *Well 1 : 5µL of BBa_K1155000 digested by SpeI | ||
| + | *Well 2 : 5µL of BBa_K1155006 digested by SpeI | ||
| + | *Well 3 : 5µL of BBa_K1155005 digested by SpeI | ||
| + | *Well 4 : 5µL of BBa_K1155004 digested by SpeI | ||
| + | *Well 5 : 6µL DNA Ladder | ||
| + | *Well 6 : 5µL of BBa_K1155000 digested by EcoRI/SpeI | ||
| + | *Well 7 : 5µL of BBa_K1155006 digested by EcoRI/SpeI | ||
| + | *Well 8 : 5µL of BBa_K1155005 digested by EcoRI/SpeI | ||
| + | *Well 9 : 5µL of BBa_K1155004 digested by EcoRI/SpeI | ||
| + | *Gel : 1% | ||
| + | |} | ||
| + | |||
| + | Expected sizes : | ||
| + | |||
| + | *PSB1C33 : 2070kb | ||
| + | *NarK, NarG, NirB : 200kb | ||
| + | *Pfnr : | ||
=====3 - Electrophoresis of the digestion of Bba_K11550007 and Bba_K1155003 by XBaI and PstI===== | =====3 - Electrophoresis of the digestion of Bba_K11550007 and Bba_K1155003 by XBaI and PstI===== | ||
Revision as of 22:45, 19 August 2013
Contents |
Notebook : August 14
Lab work
A - Aerobic/Anaerobic regulation system
Objective : obtaining ...
1 - Electrophoresis to check the digestion of Bba_K1155004,Bba_K1155005, Bba_K1155006 by EcoRI and SpeI
Anaïs, Nadia
|
|
Expected sizes :
- PSB1C33 : 2070kb
- NarK, NarG, NirB : 200kb
We obtained fragments of the right size but in very few quantity. We do it again but this time we will use more quantity of enzymes AND ADN ?!
2 - Digestion of Bba_K1155000, Bba_K1155004,Bba_K1155005, Bba_K1155006 by SpeI and EcoRI/SpeI
Anaïs, Nadia
- Digestion by SpeI :
- Buffer : 2µL
- SpeI : 2µL
- ADN : 15µL
- H20 : 1µL
- Digestion by EcoRI and SpeI :
- Buffer : 3µL
- SpeI : 2µL
- EcoRI : 2µL
- ADN : 20µL
- H20 : 3µL
3 - Electrophoresis to check the digestion of Bba_K1155000, Bba_K1155004,Bba_K1155005, Bba_K1155006 by SpeI and EcoRI/SpeI=
Anaïs, Nadia
| [[]] |
|
Expected sizes :
- PSB1C33 : 2070kb
- NarK, NarG, NirB : 200kb
- Pfnr :
3 - Electrophoresis of the digestion of Bba_K11550007 and Bba_K1155003 by XBaI and PstI
Nadia
|
|
