"
Team:Paris Saclay/Notebook/August/19
From 2013.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
===='''Objective : to obtain ... '''==== | ===='''Objective : to obtain ... '''==== | ||
| - | =====1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI | + | =====1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI/PstI===== |
Nadia | Nadia | ||
| Line 18: | Line 18: | ||
* RBS-AmilCP-Term : 21.3ng/µL | * RBS-AmilCP-Term : 21.3ng/µL | ||
| - | + | {| | |
| + | | style="border:1px solid black;padding:5px;background-color:#DEDEDE;" | | ||
| + | Concentrations are very low so we try to precipitate the DNA again. | ||
| + | |} | ||
=====2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term===== | =====2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term===== | ||
Revision as of 18:56, 10 September 2013
Contents |
Notebook : August 19
Lab work
A - Aerobic/Anaerobic regulation system
Objective : to obtain ...
1 - Gel purification of the digestion of Bba_K1155003 and Bba_K1155007 by XBaI/PstI
Nadia
Protocol : Gel purification
Nanodrop :
- RBS-LacZ-Term : 59.6ng/µL
- RBS-AmilCP-Term : 21.3ng/µL
|
Concentrations are very low so we try to precipitate the DNA again. |
2 - EtOH precipitation of RBS-LacZ-Term and RBS-AmilCP-Term
Nadia, XiaoJing
Protocol : EtOH precipitation
We used 20µL of DNA.
A - Aerobic/Anaerobic regulation system / B - PCB sensor system
Objective : to obtain ...
1 - Electrophoresis gel of BphR2 produce by PCR
Damir
|
|
We obtain the BphR2 by PCR.
2 - Gel purification of BphR2
Damir
Protocol : Gel purification
Nanodrop :
- BphR2 : 57.2ng/µL
CONCLUSION
Meeting with Evry
