Team:BYU Provo/Notebook/SmallPhage/Springexp/Period2/Dailylog

5/13/13

- Purified phage stock in 5.9 T7+ Liquid Culture Phage Concentration Test #2

- Performed 5.13 Determining E coli Concentration with Spectrophotometer

- Started the registry for phage stock

5/14/13

- Started two 5mL BL21 overnight at 6:00pm

5/15/13

- Completed 5.9 T7+ Liquid Culture Phage Concentration Test #2 by doing the spot test

- Performed 5.15 Titer Test on 5.3 T7 new Phage Stock to determine phage concentration and estimate dilution for applying mutagen

- Sorted LB plates made on May 8 and threw away the ones with obvious contamination

5/16/13

- Took plates from 5.15 out of incubation at around 4:00pm

5/17/13

- Determined that all LB plates from 5.8 had contamination

- Poured new LB plates

- Made x8 top agar

5/18/13

- Stacked up the LB plates made yesterday. No obvious sign of contamination seen.

- Threw away the 5.8 LB plates (the ones with contamination).

5/19/13

- Started two 5mL of E coli BL21 overnight

- Designed procedure for applying mutagen and selecting for T7

5/20/13

- Started T7 Mutagen Concentration Test